Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 319053..319639 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name doc Uniprot ID A0A2W7TCK5
Locus tag LB484_RS01465 Protein ID WP_008806973.1
Coordinates 319271..319639 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LB484_RS01460 Protein ID WP_004174006.1
Coordinates 319053..319274 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS01440 (LB484_01440) 315210..316136 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LB484_RS01445 (LB484_01445) 316133..317410 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
LB484_RS01450 (LB484_01450) 317407..318174 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LB484_RS01455 (LB484_01455) 318176..318889 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LB484_RS01460 (LB484_01460) 319053..319274 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LB484_RS01465 (LB484_01465) 319271..319639 + 369 WP_008806973.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LB484_RS01470 (LB484_01470) 319916..321232 + 1317 WP_008806974.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LB484_RS01475 (LB484_01475) 321339..322226 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LB484_RS01480 (LB484_01480) 322223..323068 + 846 WP_008806976.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LB484_RS01485 (LB484_01485) 323070..324140 + 1071 WP_023323353.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 316133..324877 8744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13639.01 Da        Isoelectric Point: 7.3191

>T217335 WP_008806973.1 NZ_CP083790:319271-319639 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T217335 NZ_CP114160:1952469-1952571 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT217335 WP_004174006.1 NZ_CP083790:319053-319274 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT217335 NZ_CP114160:c1952575-1952428 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W7TCK5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References