Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 277802..278433 Replicon chromosome
Accession NZ_CP083790
Organism Klebsiella variicola strain INF352

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag LB484_RS01255 Protein ID WP_044650325.1
Coordinates 277802..278077 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag LB484_RS01260 Protein ID WP_004181489.1
Coordinates 278074..278433 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB484_RS01235 (LB484_01235) 273038..273373 + 336 WP_002921203.1 universal stress protein UspB -
LB484_RS01240 (LB484_01240) 273433..274929 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
LB484_RS01245 (LB484_01245) 275159..276352 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
LB484_RS01250 (LB484_01250) 276392..277405 - 1014 WP_022066424.1 magnesium transporter -
LB484_RS01255 (LB484_01255) 277802..278077 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
LB484_RS01260 (LB484_01260) 278074..278433 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LB484_RS01265 (LB484_01265) 278461..278862 - 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
LB484_RS01270 (LB484_01270) 278850..279641 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
LB484_RS01275 (LB484_01275) 279638..280402 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
LB484_RS01280 (LB484_01280) 280402..281235 - 834 WP_012540374.1 nickel ABC transporter permease subunit NikC -
LB484_RS01285 (LB484_01285) 281232..282176 - 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T217334 WP_044650325.1 NZ_CP083790:277802-278077 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T217334 NZ_CP114160:c1789647-1789294 [Klebsiella quasipneumoniae]
ATGTGGACAATTAAAACAACGGATACGTTTGATCGCTGGTTTGCCTCGCTCAGCGATACCGACAGGGTCAGCATGCTTGC
AGCACTGCTGGTCCTGCGGGAAAAGGGACCAGGTTTGTCCAGACCTTATGCCGATACGGTCAGAGGTTCCCGCTACAGCA
ACATGAAAGAGCTACGTATTCAGAGTCGGGGCGATCCGATACGGGCGTTTTTCGCTTTTGACCCGACCCGTACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGACAGGGAATTCAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT217334 WP_004181489.1 NZ_CP083790:278074-278433 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT217334 NZ_CP114160:c1789289-1788987 [Klebsiella quasipneumoniae]
GTGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAATCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCAACGGACAT
ACTGCTCAACATTCACCTTGCCGAACTGCGTGAGAAAGTACAGAAAACGCAGGTGGAGATGGCACAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACCAGGGCGCGATCTGAAGCTTTCCACTCTCAAACGCTACGTTGAAGCGACT
GGCGGCAAGCTACGTCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTTGTACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References