Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5144605..5145230 Replicon chromosome
Accession NZ_CP083784
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LD127_RS25275 Protein ID WP_004187928.1
Coordinates 5144605..5144988 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LD127_RS25280 Protein ID WP_004150355.1
Coordinates 5144988..5145230 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS25260 (5141971) 5141971..5142873 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LD127_RS25265 (5142870) 5142870..5143505 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LD127_RS25270 (5143502) 5143502..5144431 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LD127_RS25275 (5144605) 5144605..5144988 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LD127_RS25280 (5144988) 5144988..5145230 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LD127_RS25285 (5145424) 5145424..5146341 + 918 WP_004187929.1 alpha/beta hydrolase -
LD127_RS25290 (5146355) 5146355..5147296 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LD127_RS25295 (5147341) 5147341..5147778 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LD127_RS25300 (5147775) 5147775..5148635 - 861 WP_004146232.1 virulence factor BrkB family protein -
LD127_RS25305 (5148629) 5148629..5149228 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T217332 WP_004187928.1 NZ_CP083784:c5144988-5144605 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217332 NZ_CP114157:c146271-145855 [Klebsiella michiganensis]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCTCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217332 WP_004150355.1 NZ_CP083784:c5145230-5144988 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217332 NZ_CP114157:c146498-146268 [Klebsiella michiganensis]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References