Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4534123..4534933 Replicon chromosome
Accession NZ_CP083784
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LD127_RS22360 Protein ID WP_023279404.1
Coordinates 4534123..4534656 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LD127_RS22365 Protein ID WP_002887278.1
Coordinates 4534667..4534933 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS22355 (4532954) 4532954..4534075 + 1122 WP_002887282.1 cupin domain-containing protein -
LD127_RS22360 (4534123) 4534123..4534656 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LD127_RS22365 (4534667) 4534667..4534933 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LD127_RS22370 (4535036) 4535036..4536469 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LD127_RS22375 (4536459) 4536459..4537142 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LD127_RS22380 (4537314) 4537314..4538699 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LD127_RS22385 (4538717) 4538717..4539061 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T217328 WP_023279404.1 NZ_CP083784:c4534656-4534123 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T217328 NZ_CP114156:c4675334-4675077 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT217328 WP_002887278.1 NZ_CP083784:c4534933-4534667 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT217328 NZ_CP114156:c4675066-4674515 [Klebsiella michiganensis]
ATGACAAACCATAGCTTCACTATTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGATGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCTGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References