Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2562344..2563932 Replicon chromosome
Accession NZ_CP083784
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name hipA Uniprot ID A0A486KW17
Locus tag LD127_RS12605 Protein ID WP_004190166.1
Coordinates 2562344..2563666 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag LD127_RS12610 Protein ID WP_002904790.1
Coordinates 2563666..2563932 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS12590 (2557400) 2557400..2559901 + 2502 WP_004190161.1 fimbria/pilus outer membrane usher protein -
LD127_RS12595 (2559889) 2559889..2560893 + 1005 WP_004190163.1 fimbrial protein -
LD127_RS12600 (2561035) 2561035..2562327 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
LD127_RS12605 (2562344) 2562344..2563666 - 1323 WP_004190166.1 type II toxin-antitoxin system HipA family toxin Toxin
LD127_RS12610 (2563666) 2563666..2563932 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LD127_RS12615 (2564197) 2564197..2564523 - 327 WP_004143476.1 hydroxyisourate hydrolase -
LD127_RS12620 (2564520) 2564520..2565020 - 501 WP_004190169.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LD127_RS12625 (2564998) 2564998..2566377 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
LD127_RS12630 (2566723) 2566723..2567877 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
LD127_RS12635 (2567858) 2567858..2568781 - 924 WP_004190172.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T217326 WP_004190166.1 NZ_CP083784:c2563666-2562344 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T217326 NZ_CP114156:2153788-2153891 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT217326 WP_002904790.1 NZ_CP083784:c2563932-2563666 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT217326 NZ_CP114156:c2153898-2153730 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486KW17

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References