Detailed information of TA system TA217312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 81082..81818
• Replicon: plasmid pINF118_1
• Accession: NZ_CP083776
• Organism: Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LB478_RS27595
• Protein ID: WP_003026803.1
• Coordinates: 81336..81818 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LB478_RS27590
• Protein ID: WP_003026799.1
• Coordinates: 81082..81348 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB478_RS27565 (LB478_27565)	76367..76771	-	405	WP_003031976.1	transposase	-
LB478_RS27570 (LB478_27570)	77574..77882	-	309	WP_017896554.1	hypothetical protein	-
LB478_RS27580 (LB478_27580)	79486..80466	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
LB478_RS27585 (LB478_27585)	80520..80837	+	318	WP_224440782.1	cell envelope integrity protein TolA	-
LB478_RS27590 (LB478_27590)	81082..81348	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LB478_RS27595 (LB478_27595)	81336..81818	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LB478_RS27600 (LB478_27600)	82019..83422	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LB478_RS27605 (LB478_27605)	83451..84083	-	633	WP_001567369.1	hypothetical protein	-
LB478_RS27610 (LB478_27610)	84607..85611	-	1005	WP_000427620.1	IS110-like element IS4321 family transposase	-
LB478_RS27615 (LB478_27615)	85690..86247	-	558	WP_003100847.1	recombinase family protein	-
LB478_RS27620 (LB478_27620)	86241..86612	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..256803	256803
	-	inside	IScluster/Tn	-	-	69600..92932	23332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T217312 WP_003026803.1 NZ_CP083776:81336-81818 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T217312 NZ_CP114149:841405-841815 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAACTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACTCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT217312 WP_003026799.1 NZ_CP083776:81082-81348 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT217312 NZ_CP114149:841158-841424 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTCAGCGATGGCGACAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66