Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42451..43094 Replicon plasmid pINF118_1
Accession NZ_CP083776
Organism Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LB478_RS27400 Protein ID WP_001044770.1
Coordinates 42678..43094 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LB478_RS27395 Protein ID WP_001261282.1
Coordinates 42451..42681 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB478_RS27360 (LB478_27360) 38475..39443 + 969 WP_224440781.1 IS5-like element IS903B family transposase -
LB478_RS27365 (LB478_27365) 39485..39658 - 174 WP_157847285.1 hypothetical protein -
LB478_RS27370 (LB478_27370) 39980..40510 + 531 WP_223174905.1 hypothetical protein -
LB478_RS27375 (LB478_27375) 40479..40916 + 438 WP_009309910.1 hypothetical protein -
LB478_RS27380 (LB478_27380) 41107..41547 - 441 WP_047057603.1 hypothetical protein -
LB478_RS27385 (LB478_27385) 41566..42189 - 624 WP_223345396.1 hypothetical protein -
LB478_RS27390 (LB478_27390) 42258..42494 - 237 Protein_45 hypothetical protein -
LB478_RS27395 (LB478_27395) 42451..42681 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LB478_RS27400 (LB478_27400) 42678..43094 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LB478_RS27405 (LB478_27405) 43168..44730 + 1563 WP_009309907.1 AAA family ATPase -
LB478_RS27410 (LB478_27410) 44715..45737 + 1023 WP_000361404.1 helicase UvrD -
LB478_RS27415 (LB478_27415) 46281..47189 + 909 WP_032425603.1 HNH endonuclease -
LB478_RS27420 (LB478_27420) 47375..47725 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..256803 256803


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T217311 WP_001044770.1 NZ_CP083776:42678-43094 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T217311 NZ_CP114149:c57079-56771 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTAATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT217311 WP_001261282.1 NZ_CP083776:42451-42681 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT217311 NZ_CP114149:c57318-57082 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTTAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References