Detailed information of TA system TA217310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19091..19734
• Replicon: plasmid pINF118_1
• Accession: NZ_CP083776
• Organism: Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7Z7T700
• Locus tag: LB478_RS27275
• Protein ID: WP_040155119.1
• Coordinates: 19318..19734 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LB478_RS27270
• Protein ID: WP_001261276.1
• Coordinates: 19091..19321 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB478_RS27235 (LB478_27235)	14370..15005	+	636	WP_000677445.1	type 3 fimbria minor subunit MrkF	-
LB478_RS27240 (LB478_27240)	15040..15756	-	717	WP_004149658.1	phosphodiesterase MrkJ	-
LB478_RS27245 (LB478_27245)	16090..17070	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
LB478_RS27250 (LB478_27250)	17249..17584	-	336	WP_009309920.1	hypothetical protein	-
LB478_RS27255 (LB478_27255)	17634..17945	-	312	WP_048321656.1	hypothetical protein	-
LB478_RS27260 (LB478_27260)	18003..18308	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
LB478_RS27265 (LB478_27265)	18310..18528	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
LB478_RS27270 (LB478_27270)	19091..19321	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LB478_RS27275 (LB478_27275)	19318..19734	+	417	WP_040155119.1	type II toxin-antitoxin system VapC family toxin	Toxin
LB478_RS27280 (LB478_27280)	19775..20653	-	879	WP_009309916.1	restriction endonuclease	-
LB478_RS27285 (LB478_27285)	21315..22592	+	1278	WP_048321652.1	HlyD family secretion protein	-
LB478_RS27290 (LB478_27290)	22582..24690	+	2109	WP_032427226.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..256803	256803
	-	flank	IS/Tn	-	-	16090..17070	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15147.60 Da
• Isoelectric Point: 7.8915

>T217310 WP_040155119.1 NZ_CP083776:19318-19734 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T217310 NZ_CP114134:c4069595-4069314 [Xanthomonas fragariae]
ATGATCAGGAGCTTTGTCGACAAGGAAGCCGAAAAGATCTGGATGGGTGAGCGCTCCCGCCGGTTGCCGGCCGACATCCA
GTCGGTCGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTCGACGATCTGCGCATTCCGCCTGCCAACCGGC
TGGAAGCGTTGAAGGGCGAGCGGCGCGGGCAGTACAGCATCCGGATCAACGACCAGTGGCGCATCTGCTTCCGATGGATG
GAGGGCGATGTCGCCGAGGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT217310 WP_001261276.1 NZ_CP083776:19091-19321 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT217310 NZ_CP114134:c4069295-4069005 [Xanthomonas fragariae]
ATGACAGTCCTACCCAACATCCACCCTGGTGAAATCCTGCTTGAGGAGTTCCTCGAGCCGATGGGCATCAGCCAGAACGC
ATTGGCGCGGGCCACCGGTGTGCCGCCACGCCGTATCAACGAAATCGTGTTGGGCAAGCGCGGGATCACCGCCGACACCG
CCGTGCGCCTGGCTGCAGCGCTGGGCACGACCGAGCGCTTCTGGCTAGGCCTGCAAGCCGATTACGAGCTGGAACAAGCG
CACCGCGCGCTGGGCGACCTGCCATCGCGGATCAAGCGGCTGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A7Z7T700

Antitoxin

• AlphaFold DB: A0A387K023