Detailed information of TA system TA217309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 18003..18528
• Replicon: plasmid pINF118_1
• Accession: NZ_CP083776
• Organism: Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: LB478_RS27260
• Protein ID: WP_004197633.1
• Coordinates: 18003..18308 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: LB478_RS27265
• Protein ID: WP_006788213.1
• Coordinates: 18310..18528 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB478_RS27230 (LB478_27230)	13361..14356	+	996	WP_049087407.1	type 3 fimbria adhesin subunit MrkD	-
LB478_RS27235 (LB478_27235)	14370..15005	+	636	WP_000677445.1	type 3 fimbria minor subunit MrkF	-
LB478_RS27240 (LB478_27240)	15040..15756	-	717	WP_004149658.1	phosphodiesterase MrkJ	-
LB478_RS27245 (LB478_27245)	16090..17070	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
LB478_RS27250 (LB478_27250)	17249..17584	-	336	WP_009309920.1	hypothetical protein	-
LB478_RS27255 (LB478_27255)	17634..17945	-	312	WP_048321656.1	hypothetical protein	-
LB478_RS27260 (LB478_27260)	18003..18308	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
LB478_RS27265 (LB478_27265)	18310..18528	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LB478_RS27270 (LB478_27270)	19091..19321	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
LB478_RS27275 (LB478_27275)	19318..19734	+	417	WP_040155119.1	type II toxin-antitoxin system VapC family toxin	-
LB478_RS27280 (LB478_27280)	19775..20653	-	879	WP_009309916.1	restriction endonuclease	-
LB478_RS27285 (LB478_27285)	21315..22592	+	1278	WP_048321652.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..256803	256803
	-	flank	IS/Tn	-	-	16090..17070	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T217309 WP_004197633.1 NZ_CP083776:c18308-18003 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T217309 NZ_CP114134:4054552-4054968 [Xanthomonas fragariae]
GTGATCCTGCTCGATACCAATGTCATCTCCGAGCTTTGGCGGCCTCAGCCCAACCCGAAGGTCGTCGCCTGGATCGATGC
GCAGGCTGTCGAGACACTCTTCCTGTCGGTCGTGACTGTGGCCGAATTGCGTTTCGGCATTGCCGTGATGCCCGAGGGCC
GCAAGCGTTACACGTTGCATGCTCGGCTGGAAAGCGATGTGTTGCCGTTGTTCGACGGGCGTTTGCTGGCGTTCGACCTG
GACGCATCGCACGCATTTGCGACACTTGCATCGAAAGCCAGAACCGCAGGACTCACACTCGGGCGCGCCGATGCTTACAT
CGCTGCAACGGCAGCCGCGCACGCATTGGCTGTTGCCACCCGTGATACGGCACCGTTCGAGGCGATGGCGTTGGACGTCA
TTAACCCGTGGATTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT217309 WP_006788213.1 NZ_CP083776:c18528-18310 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT217309 NZ_CP114134:4054301-4054555 [Xanthomonas fragariae]
ATGCCTTCTTTCACCGTCCGCAACATTCCCGACGACGTGCATCGCGCGATTCGCGCGAGGGCCGCACTCCACGGCCGCAG
CACCGAGGCGGAGATCCGCGCAATCCTTGAATCAGCCGCCAAACCAGCCGGCCGGGTCAAACTCGGCTCCCTGCTGGTGG
ACATCGGCCGCGACGCTGGCCTGACAGCAAAAGAGGCCAACGCCTTCGACACGATGCGTGATAAGGCCGCTATCAAGCCC
CTCGGTCTGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5

Antitoxin

• AlphaFold DB: A0A1D8K3R4