Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4806097..4806907 Replicon chromosome
Accession NZ_CP083775
Organism Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LB478_RS24030 Protein ID WP_004178461.1
Coordinates 4806097..4806630 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LB478_RS24035 Protein ID WP_002887278.1
Coordinates 4806641..4806907 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB478_RS24025 (LB478_24025) 4804928..4806049 + 1122 WP_004186850.1 cupin domain-containing protein -
LB478_RS24030 (LB478_24030) 4806097..4806630 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LB478_RS24035 (LB478_24035) 4806641..4806907 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LB478_RS24040 (LB478_24040) 4807010..4808443 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
LB478_RS24045 (LB478_24045) 4808433..4809116 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LB478_RS24050 (LB478_24050) 4809289..4810674 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
LB478_RS24055 (LB478_24055) 4810692..4811036 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T217305 WP_004178461.1 NZ_CP083775:c4806630-4806097 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T217305 NZ_CP114134:1493524-1493886 [Xanthomonas fragariae]
ATGCCTGAAAAGCCTCTGATATGGGTTGGATCAAGTAGACGTGACCTGGCAGAGTTACCTGAAGACGTTAAGGATACCTT
TGGATTTGCACTGTCCGAAGCTCAGAATGGACGCACCCATGTGAGAGCAAAGCCTATGGCTAAGGGATCACTGAAGGGTA
AGGGCATCTACGAGGTGGTGGACGACTATGATGGTGATACTTTTAGGGCCGTCTATACGGTGGAAATAGAGGATGCCATT
TATGCCTTGCACTGCTTCCAAAAGAAATCTAAGAACGGCAGAGAAACTCCAAAATCAGACATCGACTTGATCGAATCTCG
GTATCAAGCCGCCTTATTGGATGCAAAAAAGGTAAAAATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT217305 WP_002887278.1 NZ_CP083775:c4806907-4806641 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT217305 NZ_CP114134:1493886-1494203 [Xanthomonas fragariae]
ATGAGCATTGAAATCGAAGTCAGCAGCGGTAACATTTACTCAGATCTTGGCTATTCAAATGCCGAGGAAATGAAAATTAA
AGCTGCGCTTGCTGGAAAGATTAGCGCATTCATTGCAGACGCACATCTCACTCAGGCTCAAGCAGCTGCCCACATGGGCA
TCTCTCAAGCAAAAGTGTCCGAAATGCTCCGGGGTCGCTTTCGCGGAATCTCCGAGGCTAAGATGATGGAATGCCTTAAG
AGCTTTGGTCAGAACGTTACAATAAGCGTCACACCGGCTGAAGTTCCTGACGAGGCGGAGATTTCTGTTGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References