Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 989883..990460 Replicon chromosome
Accession NC_008543
Organism Burkholderia cenocepacia HI2424

Toxin (Protein)


Gene name vapC Uniprot ID A0A3R9B004
Locus tag BCEN2424_RS20280 Protein ID WP_011547972.1
Coordinates 989883..990248 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B1K204
Locus tag BCEN2424_RS20285 Protein ID WP_011547971.1
Coordinates 990242..990460 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCEN2424_RS20260 985318..986217 + 900 WP_011694702.1 lipid A hydroxylase LpxO -
BCEN2424_RS20265 986374..987207 + 834 WP_011547975.1 hypothetical protein -
BCEN2424_RS20270 987303..988904 - 1602 WP_011547974.1 alkyl hydroperoxide reductase subunit F -
BCEN2424_RS20275 989023..989586 - 564 WP_011547973.1 peroxiredoxin -
BCEN2424_RS20280 989883..990248 - 366 WP_011547972.1 type II toxin-antitoxin system VapC family toxin Toxin
BCEN2424_RS20285 990242..990460 - 219 WP_011547971.1 hypothetical protein Antitoxin
BCEN2424_RS20290 990552..991448 - 897 WP_011547970.1 LysR family transcriptional regulator -
BCEN2424_RS20295 991629..992795 + 1167 WP_011547969.1 branched-chain amino acid ABC transporter substrate-binding protein -
BCEN2424_RS20300 992837..993361 + 525 WP_011547968.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
BCEN2424_RS20305 993354..994610 + 1257 WP_011547967.1 Zn-dependent hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13396.44 Da        Isoelectric Point: 8.4487

>T21730 WP_011547972.1 NC_008543:c990248-989883 [Burkholderia cenocepacia HI2424]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISMISWMEVLVGASASDEAPIRAWLSSFNVIALDSTVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPASEPGVRVPYQI

Download         Length: 366 bp

>T21730 NC_008543:c990248-989883 [Burkholderia cenocepacia HI2424]
ATGGTAAAAGCCCTTTTCGACACCAACATCCTGATCGATTATCTGGGCGGCGTGGAATCCGCGCGTGCGGAGCTAGGCCG
CTACGACTATCGCGCAATCAGCATGATCTCGTGGATGGAAGTCCTCGTCGGGGCGTCCGCAAGTGACGAGGCCCCCATCC
GTGCATGGCTCTCGTCGTTCAATGTCATCGCGCTCGACAGCACGGTGGCGAATCGCGCCGTGACGATTCGCAAGGAACGC
CGCATACGTCTGCCCGACGCAATCGTGTGGGCGTCAGCACAAGTGAACGGGCTGTTGCTCGTATCGCGCAACACGAAGGA
CTTTCCCGCCTCCGAGCCCGGCGTTCGCGTGCCATATCAGATCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8292.38 Da        Isoelectric Point: 4.5560

>AT21730 WP_011547971.1 NC_008543:c990460-990242 [Burkholderia cenocepacia HI2424]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALNKHRLADDVFGLWKDRTVDGLAYQEELRSEW

Download         Length: 219 bp

>AT21730 NC_008543:c990460-990242 [Burkholderia cenocepacia HI2424]
ATGAGCCGGATTCTGGTCGATCTGTCGGATGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACACCGTCCCCG
CGCCGCCATCATTCGTGACGCGATCGACGCCTACATTGCCCTGAACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTAT
GGAAAGATCGCACGGTCGACGGGCTCGCCTATCAAGAGGAATTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9B004

Antitoxin

Source ID Structure
AlphaFold DB B1K204

References