Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1545004..1545740 Replicon chromosome
Accession NZ_CP083775
Organism Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LB478_RS07870 Protein ID WP_003026803.1
Coordinates 1545258..1545740 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LB478_RS07865 Protein ID WP_003026799.1
Coordinates 1545004..1545270 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB478_RS07835 (LB478_07835) 1540710..1540992 - 283 Protein_1532 hypothetical protein -
LB478_RS07840 (LB478_07840) 1541106..1542086 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
LB478_RS07845 (LB478_07845) 1542392..1543426 + 1035 WP_015065590.1 hypothetical protein -
LB478_RS07850 (LB478_07850) 1543560..1544051 + 492 WP_015065591.1 hypothetical protein -
LB478_RS07855 (LB478_07855) 1544112..1544315 + 204 WP_004213596.1 HHA domain-containing protein -
LB478_RS07860 (LB478_07860) 1544329..1544559 + 231 WP_004206660.1 hypothetical protein -
LB478_RS07865 (LB478_07865) 1545004..1545270 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LB478_RS07870 (LB478_07870) 1545258..1545740 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LB478_RS07875 (LB478_07875) 1545899..1547005 - 1107 Protein_1540 IS3-like element ISKpn38 family transposase -
LB478_RS07880 (LB478_07880) 1547153..1548133 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
LB478_RS07885 (LB478_07885) 1548744..1550231 + 1488 WP_162898809.1 group II intron reverse transcriptase/maturase -
LB478_RS07890 (LB478_07890) 1550309..1550548 + 240 WP_213491209.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 1538355..1548133 9778


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T217294 WP_003026803.1 NZ_CP083775:1545258-1545740 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T217294 NZ_CP114130:3727958-3728176 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT217294 WP_003026799.1 NZ_CP083775:1545004-1545270 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT217294 NZ_CP114130:3727558-3727932 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References