Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28895..29538 Replicon plasmid pKSB1_9E_1
Accession NZ_CP083769
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LB480_RS25905 Protein ID WP_001044770.1
Coordinates 29122..29538 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LB480_RS25900 Protein ID WP_001261282.1
Coordinates 28895..29125 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS25860 (LB480_25860) 24425..25015 + 591 Protein_28 ATP-binding cassette domain-containing protein -
LB480_RS25865 (LB480_25865) 25015..25650 + 636 WP_009309912.1 type II CAAX endopeptidase family protein -
LB480_RS25870 (LB480_25870) 25758..26102 - 345 WP_009309911.1 hypothetical protein -
LB480_RS25875 (LB480_25875) 26424..26954 + 531 WP_223174905.1 hypothetical protein -
LB480_RS25880 (LB480_25880) 26923..27360 + 438 WP_009309910.1 hypothetical protein -
LB480_RS25885 (LB480_25885) 27551..27991 - 441 WP_047057603.1 hypothetical protein -
LB480_RS25890 (LB480_25890) 28010..28633 - 624 WP_223345396.1 hypothetical protein -
LB480_RS25895 (LB480_25895) 28702..28938 - 237 Protein_35 hypothetical protein -
LB480_RS25900 (LB480_25900) 28895..29125 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LB480_RS25905 (LB480_25905) 29122..29538 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LB480_RS25910 (LB480_25910) 29612..31174 + 1563 WP_009309907.1 AAA family ATPase -
LB480_RS25915 (LB480_25915) 31159..32181 + 1023 WP_000361404.1 helicase UvrD -
LB480_RS25920 (LB480_25920) 32725..33633 + 909 WP_032425603.1 HNH endonuclease -
LB480_RS25925 (LB480_25925) 33819..34169 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..250893 250893
- flank IS/Tn - - 23312..24292 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T217289 WP_001044770.1 NZ_CP083769:29122-29538 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T217289 NZ_CP114130:2581688-2581795 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT217289 WP_001261282.1 NZ_CP083769:28895-29125 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT217289 NZ_CP114130:c2581635-2581574 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References