Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18216..18859 Replicon plasmid pKSB1_9E_1
Accession NZ_CP083769
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag LB480_RS25835 Protein ID WP_020804312.1
Coordinates 18443..18859 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LB480_RS25830 Protein ID WP_001261276.1
Coordinates 18216..18446 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS25795 (LB480_25795) 13998..14255 + 258 WP_009310077.1 hypothetical protein -
LB480_RS25800 (LB480_25800) 14313..15092 - 780 WP_023287113.1 site-specific integrase -
LB480_RS25805 (LB480_25805) 15290..16306 - 1017 WP_009309921.1 hypothetical protein -
LB480_RS25810 (LB480_25810) 16340..16675 - 336 WP_009309920.1 hypothetical protein -
LB480_RS25815 (LB480_25815) 16725..16865 - 141 WP_162898808.1 hypothetical protein -
LB480_RS25820 (LB480_25820) 17094..17399 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB -
LB480_RS25825 (LB480_25825) 17401..17619 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
LB480_RS25830 (LB480_25830) 18216..18446 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LB480_RS25835 (LB480_25835) 18443..18859 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
LB480_RS25840 (LB480_25840) 18900..19778 - 879 WP_032427228.1 restriction endonuclease -
LB480_RS25845 (LB480_25845) 20440..21717 + 1278 WP_009309915.1 HlyD family secretion protein -
LB480_RS25850 (LB480_25850) 21707..23224 + 1518 Protein_26 ABC transporter transmembrane domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..250893 250893
- flank IS/Tn - - 23312..24292 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T217288 WP_020804312.1 NZ_CP083769:18443-18859 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T217288 NZ_CP114130:2581152-2581259 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT217288 WP_001261276.1 NZ_CP083769:18216-18446 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT217288 NZ_CP114130:c2581104-2581045 [Escherichia coli]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References