Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17094..17619 Replicon plasmid pKSB1_9E_1
Accession NZ_CP083769
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag LB480_RS25820 Protein ID WP_009309918.1
Coordinates 17094..17399 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag LB480_RS25825 Protein ID WP_006788213.1
Coordinates 17401..17619 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS25790 (LB480_25790) 12299..13891 + 1593 WP_039264109.1 IS66 family transposase -
LB480_RS25795 (LB480_25795) 13998..14255 + 258 WP_009310077.1 hypothetical protein -
LB480_RS25800 (LB480_25800) 14313..15092 - 780 WP_023287113.1 site-specific integrase -
LB480_RS25805 (LB480_25805) 15290..16306 - 1017 WP_009309921.1 hypothetical protein -
LB480_RS25810 (LB480_25810) 16340..16675 - 336 WP_009309920.1 hypothetical protein -
LB480_RS25815 (LB480_25815) 16725..16865 - 141 WP_162898808.1 hypothetical protein -
LB480_RS25820 (LB480_25820) 17094..17399 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
LB480_RS25825 (LB480_25825) 17401..17619 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LB480_RS25830 (LB480_25830) 18216..18446 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
LB480_RS25835 (LB480_25835) 18443..18859 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
LB480_RS25840 (LB480_25840) 18900..19778 - 879 WP_032427228.1 restriction endonuclease -
LB480_RS25845 (LB480_25845) 20440..21717 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..250893 250893


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T217287 WP_009309918.1 NZ_CP083769:c17399-17094 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217287 NZ_CP114130:2533501-2533779 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT217287 WP_006788213.1 NZ_CP083769:c17619-17401 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217287 NZ_CP114130:2533308-2533499 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References