Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5167196..5167821 Replicon chromosome
Accession NZ_CP083768
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LB480_RS25355 Protein ID WP_002882817.1
Coordinates 5167196..5167579 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LB480_RS25360 Protein ID WP_004150355.1
Coordinates 5167579..5167821 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS25340 (5164562) 5164562..5165464 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LB480_RS25345 (5165461) 5165461..5166096 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LB480_RS25350 (5166093) 5166093..5167022 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LB480_RS25355 (5167196) 5167196..5167579 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LB480_RS25360 (5167579) 5167579..5167821 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LB480_RS25365 (5168026) 5168026..5168943 + 918 WP_060415997.1 alpha/beta hydrolase -
LB480_RS25370 (5168957) 5168957..5169898 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LB480_RS25375 (5169943) 5169943..5170380 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LB480_RS25380 (5170377) 5170377..5171237 - 861 WP_004146232.1 virulence factor BrkB family protein -
LB480_RS25385 (5171231) 5171231..5171830 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T217286 WP_002882817.1 NZ_CP083768:c5167579-5167196 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217286 NZ_CP114130:c2481688-2481533 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217286 WP_004150355.1 NZ_CP083768:c5167821-5167579 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217286 NZ_CP114130:2481700-2481758 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References