Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3974613..3975232 Replicon chromosome
Accession NZ_CP083768
Organism Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LB480_RS19655 Protein ID WP_002892050.1
Coordinates 3975014..3975232 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LB480_RS19650 Protein ID WP_002892066.1
Coordinates 3974613..3974987 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB480_RS19640 (3969765) 3969765..3970958 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LB480_RS19645 (3970981) 3970981..3974127 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LB480_RS19650 (3974613) 3974613..3974987 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LB480_RS19655 (3975014) 3975014..3975232 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LB480_RS19660 (3975391) 3975391..3975957 + 567 WP_002892030.1 maltose O-acetyltransferase -
LB480_RS19665 (3975929) 3975929..3976069 - 141 WP_004147370.1 hypothetical protein -
LB480_RS19670 (3976090) 3976090..3976560 + 471 WP_002892026.1 YlaC family protein -
LB480_RS19675 (3976535) 3976535..3977986 - 1452 WP_040236504.1 PLP-dependent aminotransferase family protein -
LB480_RS19680 (3978087) 3978087..3978785 + 699 Protein_3857 GNAT family protein -
LB480_RS19685 (3978782) 3978782..3978922 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LB480_RS19690 (3978922) 3978922..3979185 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217282 WP_002892050.1 NZ_CP083768:3975014-3975232 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T217282 NZ_CP114130:1779858-1779961 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT217282 WP_002892066.1 NZ_CP083768:3974613-3974987 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT217282 NZ_CP114130:c1779999-1779728 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References