Detailed information of TA system TA021728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 898182..898714
• Replicon: chromosome
• Accession: NC_008542
• Organism: Burkholderia cenocepacia HI2424

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1JWG6
• Locus tag: BCEN2424_RS03920
• Protein ID: WP_011544674.1
• Coordinates: 898182..898505 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BCEN2424_RS34740
• Protein ID: WP_011544675.1
• Coordinates: 898502..898714 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCEN2424_RS03895	893193..894026	-	834	WP_011544669.1	class II glutamine amidotransferase	-
BCEN2424_RS03900	894225..894554	-	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
BCEN2424_RS03905	894684..895631	+	948	WP_011544670.1	AraC family transcriptional regulator	-
BCEN2424_RS03910	895735..896178	+	444	WP_011544671.1	YiaA/YiaB family protein	-
BCEN2424_RS34730	896301..897335	-	1035	WP_011694170.1	relaxase/mobilization nuclease domain-containing protein	-
BCEN2424_RS34735	897337..897861	-	525	WP_011694171.1	hypothetical protein	-
BCEN2424_RS03920	898182..898505	-	324	WP_011544674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCEN2424_RS34740	898502..898714	-	213	WP_011544675.1	antitoxin MazE family protein	Antitoxin
BCEN2424_RS03925	898827..899306	-	480	WP_011694172.1	hypothetical protein	-
BCEN2424_RS03930	899374..899844	-	471	WP_011544677.1	hypothetical protein	-
BCEN2424_RS03935	899877..900227	+	351	WP_011544678.1	helix-turn-helix domain-containing protein	-
BCEN2424_RS03945	900656..901405	-	750	WP_011544679.1	tol-pal system protein YbgF	-
BCEN2424_RS03950	901425..901937	-	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
BCEN2424_RS03955	901955..903250	-	1296	WP_006476798.1	Tol-Pal system protein TolB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11668.77 Da
• Isoelectric Point: 8.9366

>T21728 WP_011544674.1 NC_008542:c898505-898182 [Burkholderia cenocepacia HI2424]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T21728 NC_008542:c898505-898182 [Burkholderia cenocepacia HI2424]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8026.00 Da
• Isoelectric Point: 6.2095

>AT21728 WP_011544675.1 NC_008542:c898714-898502 [Burkholderia cenocepacia HI2424]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRRPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT21728 NC_008542:c898714-898502 [Burkholderia cenocepacia HI2424]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTGCCCGACGTCCGCCGGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B1JWG6