Detailed information of TA system TA217269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1288432..1289349
• Replicon: chromosome
• Accession: NZ_CP083764
• Organism: Bacillus velezensis strain DMB07

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: LAZ98_RS06815
• Protein ID: WP_007407256.1
• Coordinates: 1288603..1289349 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LAZ98_RS06810
• Protein ID: WP_003154807.1
• Coordinates: 1288432..1288602 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAZ98_RS06760 (1283639)	1283639..1284151	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
LAZ98_RS06765 (1284264)	1284264..1284818	+	555	Protein_1268	terminase	-
LAZ98_RS06770 (1284926)	1284926..1285288	+	363	WP_014721043.1	hypothetical protein	-
LAZ98_RS06775 (1285301)	1285301..1285672	+	372	WP_017417608.1	XkdW family protein	-
LAZ98_RS06780 (1285677)	1285677..1285874	+	198	WP_007610833.1	XkdX family protein	-
LAZ98_RS06785 (1285931)	1285931..1286692	+	762	WP_025649639.1	hypothetical protein	-
LAZ98_RS06790 (1286743)	1286743..1287006	+	264	WP_003154815.1	hemolysin XhlA family protein	-
LAZ98_RS06795 (1287020)	1287020..1287283	+	264	WP_003154813.1	phage holin	-
LAZ98_RS06800 (1287297)	1287297..1288175	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
LAZ98_RS06805 (1288210)	1288210..1288335	-	126	WP_003154809.1	hypothetical protein	-
LAZ98_RS06810 (1288432)	1288432..1288602	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LAZ98_RS06815 (1288603)	1288603..1289349	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LAZ98_RS06820 (1289453)	1289453..1290451	-	999	WP_017417610.1	inorganic phosphate transporter	-
LAZ98_RS06825 (1290464)	1290464..1291081	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LAZ98_RS06830 (1291367)	1291367..1292683	-	1317	WP_007610842.1	amino acid permease	-
LAZ98_RS06835 (1293005)	1293005..1293955	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T217269 WP_007407256.1 NZ_CP083764:c1289349-1288603 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T217269 NZ_CP114128:3077714-3078001 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT217269 WP_003154807.1 NZ_CP083764:c1288602-1288432 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT217269 NZ_CP114128:3077523-3077714 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14