Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 488485..489122 Replicon chromosome
Accession NZ_CP083764
Organism Bacillus velezensis strain DMB07

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LAZ98_RS02490 Protein ID WP_003156187.1
Coordinates 488772..489122 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LAZ98_RS02485 Protein ID WP_003156188.1
Coordinates 488485..488766 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAZ98_RS02465 (484850) 484850..485449 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
LAZ98_RS02470 (485542) 485542..485907 + 366 WP_007609580.1 holo-ACP synthase -
LAZ98_RS02475 (486072) 486072..487079 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
LAZ98_RS02480 (487196) 487196..488365 + 1170 WP_224443522.1 alanine racemase -
LAZ98_RS02485 (488485) 488485..488766 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LAZ98_RS02490 (488772) 488772..489122 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LAZ98_RS02495 (489240) 489240..490061 + 822 WP_003156182.1 STAS domain-containing protein -
LAZ98_RS02500 (490066) 490066..490431 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
LAZ98_RS02505 (490434) 490434..490835 + 402 WP_003156178.1 anti-sigma regulatory factor -
LAZ98_RS02510 (490847) 490847..491854 + 1008 WP_224443523.1 PP2C family protein-serine/threonine phosphatase -
LAZ98_RS02515 (491918) 491918..492247 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LAZ98_RS02520 (492244) 492244..492726 + 483 WP_007609591.1 anti-sigma B factor RsbW -
LAZ98_RS02525 (492692) 492692..493480 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LAZ98_RS02530 (493480) 493480..494082 + 603 WP_017419222.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T217268 WP_003156187.1 NZ_CP083764:488772-489122 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T217268 NZ_CP114128:c2909882-2909779 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT217268 WP_003156188.1 NZ_CP083764:488485-488766 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT217268 NZ_CP114128:2909741-2910012 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References