Detailed information of TA system TA217266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 493929..494566
• Replicon: chromosome
• Accession: NZ_CP083763
• Organism: Bacillus velezensis strain DMB06

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LAZ97_RS02495
• Protein ID: WP_003156187.1
• Coordinates: 494216..494566 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LAZ97_RS02490
• Protein ID: WP_003156188.1
• Coordinates: 493929..494210 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAZ97_RS02470 (LAZ97_02470)	490294..490893	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
LAZ97_RS02475 (LAZ97_02475)	490986..491351	+	366	WP_007609580.1	holo-ACP synthase	-
LAZ97_RS02480 (LAZ97_02480)	491516..492523	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
LAZ97_RS02485 (LAZ97_02485)	492640..493809	+	1170	WP_025649966.1	alanine racemase	-
LAZ97_RS02490 (LAZ97_02490)	493929..494210	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LAZ97_RS02495 (LAZ97_02495)	494216..494566	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LAZ97_RS02500 (LAZ97_02500)	494684..495505	+	822	WP_025649964.1	STAS domain-containing protein	-
LAZ97_RS02505 (LAZ97_02505)	495510..495875	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
LAZ97_RS02510 (LAZ97_02510)	495878..496279	+	402	WP_003156178.1	anti-sigma regulatory factor	-
LAZ97_RS02515 (LAZ97_02515)	496291..497298	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
LAZ97_RS02520 (LAZ97_02520)	497362..497691	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
LAZ97_RS02525 (LAZ97_02525)	497688..498170	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
LAZ97_RS02530 (LAZ97_02530)	498136..498924	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
LAZ97_RS02535 (LAZ97_02535)	498924..499526	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T217266 WP_003156187.1 NZ_CP083763:494216-494566 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T217266 NZ_CP114128:2481284-2481460 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT217266 WP_003156188.1 NZ_CP083763:493929-494210 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT217266 NZ_CP114128:2481506-2481922 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTCTTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5