Detailed information of TA system TA217263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 225058..226681
• Replicon: plasmid p1
• Accession: NZ_CP083755
• Organism: Serratia marcescens strain YL4

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: LBP97_RS25970
• Protein ID: WP_000088044.1
• Coordinates: 225058..226383 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: LBP97_RS25975
• Protein ID: WP_012477377.1
• Coordinates: 226388..226681 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBP97_RS25935 (LBP97_25975)	220967..221401	+	435	WP_000405672.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
LBP97_RS25940 (LBP97_25980)	221492..221890	+	399	Protein_248	IS110 family transposase	-
LBP97_RS25945 (LBP97_25985)	221923..222891	+	969	WP_072202995.1	IS5 family transposase	-
LBP97_RS25950 (LBP97_25990)	222947..223561	+	615	Protein_250	IS110-like element IS4321 family transposase	-
LBP97_RS25955 (LBP97_25995)	223743..224267	-	525	WP_001805097.1	hypothetical protein	-
LBP97_RS25960 (LBP97_26000)	224280..224690	-	411	WP_000985909.1	hypothetical protein	-
LBP97_RS25965 (LBP97_26005)	224791..224997	-	207	WP_000134171.1	hypothetical protein	-
LBP97_RS25970 (LBP97_26010)	225058..226383	-	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
LBP97_RS25975 (LBP97_26015)	226388..226681	-	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
LBP97_RS25980 (LBP97_26020)	227082..228470	-	1389	WP_012695445.1	hypothetical protein	-
LBP97_RS25985 (LBP97_26025)	228475..228972	-	498	WP_000340139.1	membrane protein	-
LBP97_RS25990 (LBP97_26030)	229175..229831	-	657	WP_000462754.1	trypsin-like serine protease	-
LBP97_RS25995 (LBP97_26035)	230490..230849	-	360	WP_063945302.1	hypothetical protein	-
LBP97_RS26000 (LBP97_26040)	230840..231544	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / blaTEM-1B / fosA5 / sul1 / qacE / blaIMP-26 / dfrA19 / catA2 / blaSHV-12	-	1..316459	316459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T217263 WP_000088044.1 NZ_CP083755:c226383-225058 [Serratia marcescens]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T217263 NZ_CP114128:2126154-2126309 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT217263 WP_012477377.1 NZ_CP083755:c226681-226388 [Serratia marcescens]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT217263 NZ_CP114128:c2126142-2126084 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7