Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 86625..87151 Replicon plasmid p1
Accession NZ_CP083755
Organism Serratia marcescens strain YL4

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LBP97_RS25175 Protein ID WP_000323025.1
Coordinates 86864..87151 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LBP97_RS25170 Protein ID WP_000534858.1
Coordinates 86625..86864 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBP97_RS25150 (LBP97_25185) 82953..84191 + 1239 WP_000219087.1 IS110-like element ISEsa2 family transposase -
LBP97_RS25155 (LBP97_25190) 84667..85239 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
LBP97_RS25160 (LBP97_25195) 85439..86362 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
LBP97_RS25165 (LBP97_25200) 86496..86600 - 105 Protein_93 protein YdfV -
LBP97_RS25170 (LBP97_25205) 86625..86864 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LBP97_RS25175 (LBP97_25210) 86864..87151 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LBP97_RS25180 (LBP97_25220) 87223..87381 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
LBP97_RS25185 (LBP97_25225) 87990..88310 - 321 WP_000332796.1 hypothetical protein -
LBP97_RS25190 (LBP97_25230) 88592..88795 - 204 WP_001015183.1 hypothetical protein -
LBP97_RS25195 (LBP97_25235) 88842..89192 - 351 WP_000743059.1 hypothetical protein -
LBP97_RS25200 (LBP97_25240) 89252..89854 - 603 WP_012695474.1 hypothetical protein -
LBP97_RS25205 (LBP97_25245) 89950..90894 - 945 WP_000778029.1 DUF5417 domain-containing protein -
LBP97_RS25210 (LBP97_25250) 91405..91581 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / blaTEM-1B / fosA5 / sul1 / qacE / blaIMP-26 / dfrA19 / catA2 / blaSHV-12 - 1..316459 316459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T217262 WP_000323025.1 NZ_CP083755:86864-87151 [Serratia marcescens]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T217262 NZ_CP114128:1893649-1893804 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAATCTGAGGAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT217262 WP_000534858.1 NZ_CP083755:86625-86864 [Serratia marcescens]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT217262 NZ_CP114128:c1893637-1893579 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References