Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4678038..4678660 Replicon chromosome
Accession NZ_CP083754
Organism Serratia marcescens strain YL4

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag LBP97_RS22110 Protein ID WP_004940313.1
Coordinates 4678038..4678241 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag LBP97_RS22115 Protein ID WP_004940312.1
Coordinates 4678292..4678660 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBP97_RS22080 (LBP97_22115) 4673737..4674075 + 339 WP_004940327.1 P-II family nitrogen regulator -
LBP97_RS22085 (LBP97_22120) 4674112..4675398 + 1287 WP_038628958.1 ammonium transporter AmtB -
LBP97_RS22090 (LBP97_22125) 4675489..4676352 - 864 WP_016928847.1 acyl-CoA thioesterase II -
LBP97_RS22095 (LBP97_22130) 4676584..4677075 + 492 WP_025301777.1 YbaY family lipoprotein -
LBP97_RS22100 (LBP97_22135) 4677140..4677454 - 315 WP_025301778.1 MGMT family protein -
LBP97_RS22110 (LBP97_22145) 4678038..4678241 - 204 WP_004940313.1 HHA domain-containing protein Toxin
LBP97_RS22115 (LBP97_22150) 4678292..4678660 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
LBP97_RS22120 (LBP97_22155) 4678819..4679172 - 354 WP_025301779.1 hypothetical protein -
LBP97_RS22125 (LBP97_22160) 4679581..4680294 + 714 WP_060447642.1 ABC transporter ATP-binding protein -
LBP97_RS22130 (LBP97_22165) 4680291..4681148 + 858 WP_043135664.1 metal ABC transporter permease -
LBP97_RS22135 (LBP97_22170) 4681174..4682052 + 879 WP_025301782.1 metal ABC transporter substrate-binding protein -
LBP97_RS22140 (LBP97_22175) 4682162..4682302 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
LBP97_RS22145 (LBP97_22180) 4682315..4682569 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T217261 WP_004940313.1 NZ_CP083754:c4678241-4678038 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T217261 NZ_CP114128:1874221-1874553 [Escherichia coli]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGGAAGC
TATTCTGAGCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT217261 WP_004940312.1 NZ_CP083754:c4678660-4678292 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT217261 NZ_CP114128:1873963-1874220 [Escherichia coli]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATGATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References