Detailed information of TA system TA217261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4678038..4678660
• Replicon: chromosome
• Accession: NZ_CP083754
• Organism: Serratia marcescens strain YL4

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A8B4G7F9
• Locus tag: LBP97_RS22110
• Protein ID: WP_004940313.1
• Coordinates: 4678038..4678241 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A2X2G5J9
• Locus tag: LBP97_RS22115
• Protein ID: WP_004940312.1
• Coordinates: 4678292..4678660 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBP97_RS22080 (LBP97_22115)	4673737..4674075	+	339	WP_004940327.1	P-II family nitrogen regulator	-
LBP97_RS22085 (LBP97_22120)	4674112..4675398	+	1287	WP_038628958.1	ammonium transporter AmtB	-
LBP97_RS22090 (LBP97_22125)	4675489..4676352	-	864	WP_016928847.1	acyl-CoA thioesterase II	-
LBP97_RS22095 (LBP97_22130)	4676584..4677075	+	492	WP_025301777.1	YbaY family lipoprotein	-
LBP97_RS22100 (LBP97_22135)	4677140..4677454	-	315	WP_025301778.1	MGMT family protein	-
LBP97_RS22110 (LBP97_22145)	4678038..4678241	-	204	WP_004940313.1	HHA domain-containing protein	Toxin
LBP97_RS22115 (LBP97_22150)	4678292..4678660	-	369	WP_004940312.1	Hha toxicity modulator TomB	Antitoxin
LBP97_RS22120 (LBP97_22155)	4678819..4679172	-	354	WP_025301779.1	hypothetical protein	-
LBP97_RS22125 (LBP97_22160)	4679581..4680294	+	714	WP_060447642.1	ABC transporter ATP-binding protein	-
LBP97_RS22130 (LBP97_22165)	4680291..4681148	+	858	WP_043135664.1	metal ABC transporter permease	-
LBP97_RS22135 (LBP97_22170)	4681174..4682052	+	879	WP_025301782.1	metal ABC transporter substrate-binding protein	-
LBP97_RS22140 (LBP97_22175)	4682162..4682302	-	141	WP_004940304.1	type B 50S ribosomal protein L36	-
LBP97_RS22145 (LBP97_22180)	4682315..4682569	-	255	WP_004940303.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8107.37 Da
• Isoelectric Point: 6.9756

>T217261 WP_004940313.1 NZ_CP083754:c4678241-4678038 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T217261 NZ_CP114128:1874221-1874553 [Escherichia coli]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGGAAGC
TATTCTGAGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14261.95 Da
• Isoelectric Point: 4.4314

>AT217261 WP_004940312.1 NZ_CP083754:c4678660-4678292 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Nucleotide

• Length: 369 bp

>AT217261 NZ_CP114128:1873963-1874220 [Escherichia coli]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATGATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Structures

Toxin

• AlphaFold DB: A0A8B4G7F9

Antitoxin

• AlphaFold DB: A0A2X2G5J9