Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3621060..3621661 Replicon chromosome
Accession NZ_CP083754
Organism Serratia marcescens strain YL4

Toxin (Protein)


Gene name vapC Uniprot ID A0A8B4GSJ7
Locus tag LBP97_RS17085 Protein ID WP_025304766.1
Coordinates 3621060..3621440 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8B4GSF3
Locus tag LBP97_RS17090 Protein ID WP_025304767.1
Coordinates 3621440..3621661 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBP97_RS17060 (LBP97_17085) 3616311..3617587 + 1277 Protein_3349 DUF3748 domain-containing protein -
LBP97_RS17065 (LBP97_17090) 3617618..3617965 - 348 WP_016929593.1 YceK/YidQ family lipoprotein -
LBP97_RS17070 (LBP97_17095) 3618275..3618688 + 414 WP_004933919.1 small heat shock chaperone IbpA -
LBP97_RS17075 (LBP97_17100) 3618795..3619223 + 429 WP_004933922.1 small heat shock chaperone IbpB -
LBP97_RS17080 (LBP97_17105) 3619391..3621049 + 1659 WP_238968819.1 putative transporter -
LBP97_RS17085 (LBP97_17110) 3621060..3621440 - 381 WP_025304766.1 type II toxin-antitoxin system VapC family toxin Toxin
LBP97_RS17090 (LBP97_17115) 3621440..3621661 - 222 WP_025304767.1 ribbon-helix-helix domain-containing protein Antitoxin
LBP97_RS17095 (LBP97_17120) 3621737..3622987 - 1251 WP_238968820.1 valine--pyruvate transaminase -
LBP97_RS17100 (LBP97_17125) 3623123..3625186 - 2064 WP_238968821.1 alpha-amylase -
LBP97_RS17105 (LBP97_17130) 3625383..3625535 + 153 WP_162837931.1 hypothetical protein -
LBP97_RS17110 (LBP97_17135) 3625537..3626514 - 978 WP_049204664.1 glyoxylate/hydroxypyruvate reductase GhrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-100)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14241.66 Da        Isoelectric Point: 7.3173

>T217259 WP_025304766.1 NZ_CP083754:c3621440-3621060 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T217259 NZ_CP114128:998540-998875 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8607.55 Da        Isoelectric Point: 4.4915

>AT217259 WP_025304767.1 NZ_CP083754:c3621661-3621440 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Download         Length: 222 bp

>AT217259 NZ_CP114128:998292-998540 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4GSJ7

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GSF3

References