Detailed information of TA system TA217244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 13594..14141
• Replicon: chromosome
• Accession: NZ_CP083754
• Organism: Serratia marcescens strain YL4

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A380ADA6
• Locus tag: LBP97_RS00070
• Protein ID: WP_025302144.1
• Coordinates: 13833..14141 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LBP97_RS00065
• Protein ID: WP_025302143.1
• Coordinates: 13594..13830 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBP97_RS00050 (LBP97_00050)	10080..11615	+	1536	WP_025302140.1	glutathione ABC transporter substrate-binding protein GsiB	-
LBP97_RS00055 (LBP97_00055)	11668..12588	+	921	WP_025302141.1	glutathione ABC transporter permease GsiC	-
LBP97_RS00060 (LBP97_00060)	12598..13506	+	909	WP_169540348.1	glutathione ABC transporter permease GsiD	-
LBP97_RS00065 (LBP97_00065)	13594..13830	+	237	WP_025302143.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LBP97_RS00070 (LBP97_00070)	13833..14141	+	309	WP_025302144.1	CcdB family protein	Toxin
LBP97_RS00075 (LBP97_00075)	14181..15023	-	843	WP_015377214.1	S-formylglutathione hydrolase	-
LBP97_RS00080 (LBP97_00080)	15038..16162	-	1125	WP_016928454.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
LBP97_RS00085 (LBP97_00085)	16193..17113	-	921	WP_043142419.1	LysR family transcriptional regulator	-
LBP97_RS00090 (LBP97_00090)	17219..18376	+	1158	WP_043142422.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11504.32 Da
• Isoelectric Point: 5.0196

>T217244 WP_025302144.1 NZ_CP083754:13833-14141 [Serratia marcescens]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLMIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T217244 NZ_CP114115:2643120-2643416 [Pseudomonas sp. GXZC]
ATGGAGAAGAAGACACCTCACTGCAAGCTAACAACCGTCAAGGCCCTGATTGAATCTGGGAAGGTGCGCACCACGAACTC
TGCACGAGTGGGCGCCGATGCACTTAACCTTGACCTTTCCGATATGCTGGCCGTGGTAATGGGCCTTCAACCAATGGATT
TTTATAAGAGCATGACCACCTACGCCGACCACACGATATGGCAAGACGTGTATAGGCCTCACACGAAGGCGGGTAATGTG
TACCTGAAACTCACGGTTATCGAAGACGTGCTAATCGTGTCCTTCAAGGAGCTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8967.04 Da
• Isoelectric Point: 5.0807

>AT217244 WP_025302143.1 NZ_CP083754:13594-13830 [Serratia marcescens]
MANSNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT217244 NZ_CP114115:2643419-2643820 [Pseudomonas sp. GXZC]
ATGAAATGTCCTGTTTGCGGCGCTGCGGGTTTGGTCCATGACACCCGCGATGTGCCCTATACGTACAAGGGCGAAACCAC
ATTGATTAAGAACGTGACGGGTGATTTCTGCCCGTCTTGCTCTGAGTCGGTCTTGGACCTTACGGAATCAGATCGGGTCA
TGAAGGAGATGCGCGCGTTTTCCAAGCGAACCAATGCGGACCTCGTAGACCCTGGCTACATCACGAAGGTACGGAAGAAA
CTCGACTTGGACCAACGGGAAGCCGCCGAGATATTCGGGGGAGGGGTTAATGCCTTCTCGCGCTATGAGAACGGTAAGAC
TAAACCACCGCTGGCACTCATTAAGCTTCTCAAGGTACTCGACCGACATCCTGAGTTACTCAACGAAGTCAGAACGGCTT
AG

Structures

Toxin

• AlphaFold DB: A0A380ADA6