217243

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5856..6381 Replicon plasmid pFAHZZU2591mcr-8
Accession NZ_CP083753
Organism Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag LBE00_RS26270 Protein ID WP_013023785.1
Coordinates 5856..6161 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LBE00_RS26275 Protein ID WP_001568025.1
Coordinates 6163..6381 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBE00_RS26240 (LBE00_26275) 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
LBE00_RS26245 (LBE00_26280) 2190..2492 + 303 WP_004197636.1 hypothetical protein -
LBE00_RS26250 (LBE00_26285) 2932..3726 - 795 WP_004197635.1 site-specific integrase -
LBE00_RS26255 (LBE00_26290) 3924..4940 - 1017 WP_017899884.1 hypothetical protein -
LBE00_RS26260 (LBE00_26295) 4951..5265 - 315 WP_053389906.1 hypothetical protein -
LBE00_RS26265 (LBE00_26300) 5292..5687 - 396 WP_017899885.1 hypothetical protein -
LBE00_RS26270 (LBE00_26305) 5856..6161 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
LBE00_RS26275 (LBE00_26310) 6163..6381 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LBE00_RS26280 (LBE00_26315) 6551..6814 - 264 Protein_9 hypothetical protein -
LBE00_RS26285 (LBE00_26320) 6950..7531 + 582 WP_072310991.1 hypothetical protein -
LBE00_RS26290 (LBE00_26325) 7692..8720 + 1029 WP_032445668.1 Abi family protein -
LBE00_RS26295 (LBE00_26330) 8903..9694 + 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
LBE00_RS26300 (LBE00_26335) 10136..11116 - 981 WP_072311004.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-8 / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..110627 110627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T217243 WP_013023785.1 NZ_CP083753:c6161-5856 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217243 NZ_CP114115:2196352-2196540 [Pseudomonas sp. GXZC]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCGGGCTGGGTGCTGGATCGGGTCACGGGCAGTCATCATATTTT
TACCCACTGCTATAGCCCGTACACCCTCCCTGTGCCGCATCCCAAGAAGGATTTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT217243 WP_001568025.1 NZ_CP083753:c6381-6163 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217243 NZ_CP114115:2196552-2196956 [Pseudomonas sp. GXZC]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATTCAGATCCCCGATATCCCAGGTGCGGT
CACCGCCGGGGACAGTTTCGAAGAGGCCTATAACGCTGCGGTGGAAATCGCGCACATCATGCTGCAAGAGATTGCCGCAG
AAGGCGGCCCGATTCCGATGCCGACGTCGGTAGCCAACCATCACGCGCACGAAGACTACGCCGACATGGGTTGGGGGATG
TTAGAGCTGGACATCTCGCCCTACCTGGGCAAGACCGAAAAGGTCAACGTGACTTTGCCTGGTTACGTCATCCAGCGCAT
CGACCGGTATGTGCGTGAACACAAGGTCAAGAGCCGCTCTTCCTTCCTGGCGGACGCTGCGCTGGAGAAGCTGGTGCGGT
CCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References