Detailed information of TA system TA217242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 135231..135901
• Replicon: plasmid pFAHZZU2591
• Accession: NZ_CP083752
• Organism: Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LBE00_RS25895
• Protein ID: WP_004213072.1
• Coordinates: 135231..135674 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LBE00_RS25900
• Protein ID: WP_004213073.1
• Coordinates: 135671..135901 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBE00_RS25860 (LBE00_25895)	130643..130918	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LBE00_RS25865 (LBE00_25900)	130981..131472	+	492	WP_011251327.1	DM13 domain-containing protein	-
LBE00_RS25870 (LBE00_25905)	131521..132441	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LBE00_RS25875 (LBE00_25910)	132532..132935	+	404	Protein_136	GAF domain-containing protein	-
LBE00_RS25880 (LBE00_25915)	133452..134087	-	636	WP_165452888.1	mucoid phenotype regulator RmpA2	-
LBE00_RS25885 (LBE00_25920)	134504..134808	+	305	Protein_138	transposase	-
LBE00_RS25890 (LBE00_25925)	134831..135082	-	252	WP_186987481.1	hypothetical protein	-
LBE00_RS25895 (LBE00_25930)	135231..135674	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LBE00_RS25900 (LBE00_25935)	135671..135901	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LBE00_RS25905 (LBE00_25940)	136509..137642	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LBE00_RS25910 (LBE00_25945)	137658..137951	+	294	WP_004213076.1	hypothetical protein	-
LBE00_RS25915 (LBE00_25950)	137941..138147	-	207	WP_004213077.1	hypothetical protein	-
LBE00_RS25920 (LBE00_25955)	138499..138789	+	291	WP_004213078.1	hypothetical protein	-
LBE00_RS25925 (LBE00_25960)	138779..139678	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..202973	202973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T217242 WP_004213072.1 NZ_CP083752:c135674-135231 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T217242 NZ_CP114115:c1859266-1859084 [Pseudomonas sp. GXZC]
GTGGATAGCCGATATTTGATAGGTCATATCGTTGCGGACGGTTGGTATCTGGCGCGTATCAGAGGGAGTCATCATCACTT
CAAGCACCCGACTAAGCCGGGGCTGGTGACGATTCCACATCCAAAGAAGGACCTGTTGGACAAGACCGCCAAGAGCATAT
TGAAACAAGCTCTGTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT217242 WP_004213073.1 NZ_CP083752:c135901-135671 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT217242 NZ_CP114115:c1859061-1858651 [Pseudomonas sp. GXZC]
ATGCTTTACCCGATTGCAATTTCAACCGGCGACGAGGATCACGCCTGGGGCGTGGAAGTGCCGGATATTCCTGGTTGCTA
TTCCGCCGGTGACGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATCCTGGCCGAGG
ACGGTGCACCGATTCCCAGTGCGCAGAAGGTCACCCTGCATGCCGCCAACCCCCAGTACGCAGGCTGCACCTGGGCCGTG
GTGGACATTGATGTCACCAAGTATCTGGGCAAGGCGCAGAAGCTCAACATCACGCTGCCGGGTTACCTGCTGAACCGCAT
CGATGAATATGTACTGCATCATCCGGAAGAAAAAAGCCGCTCGGGGTTTCTGGCGTCGGCGGCGCTTAAAGTGCTGCAAC
AGGAGGGGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620