Detailed information of TA system TA217240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 31924..32834
• Replicon: plasmid pFAHZZU2591
• Accession: NZ_CP083752
• Organism: Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LBE00_RS25365
• Protein ID: WP_004026354.1
• Coordinates: 32364..32834 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LBE00_RS25360
• Protein ID: WP_004026357.1
• Coordinates: 31924..32367 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBE00_RS25330 (LBE00_25365)	26942..27463	+	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-
LBE00_RS25335 (LBE00_25370)	27460..28413	+	954	Protein_28	fec operon regulator FecR	-
LBE00_RS25340 (LBE00_25375)	28500..30626	+	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LBE00_RS25345 (LBE00_25380)	30701..30820	+	120	Protein_30	Fe3+-citrate ABC transporter substrate-binding protein	-
LBE00_RS25350 (LBE00_25385)	31001..31222	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
LBE00_RS25355 (LBE00_25390)	31320..31823	+	504	Protein_32	DUF4113 domain-containing protein	-
LBE00_RS25360 (LBE00_25395)	31924..32367	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LBE00_RS25365 (LBE00_25400)	32364..32834	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LBE00_RS25370 (LBE00_25405)	33459..34156	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LBE00_RS25375 (LBE00_25410)	35542..36234	-	693	WP_237083585.1	DUF1173 family protein	-
LBE00_RS25380 (LBE00_25415)	36225..36491	-	267	WP_223175074.1	DUF1173 family protein	-
LBE00_RS25385 (LBE00_25420)	36669..37163	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..202973	202973
	-	flank	IS/Tn	-	-	33653..34156	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T217240 WP_004026354.1 NZ_CP083752:32364-32834 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T217240 NZ_CP114115:1487426-1487704 [Pseudomonas sp. GXZC]
ATGATCAGAAGCTTCAAATGCGCGGATACCAAAACCCTTTTTGAAACCGGTCGAACCCGGCGCTGGTCTGCCATCGTGTC
GGTGATTGAACGAAAGCTCAGCATGCTCGATGCGGCCATTGATCTTGCCGATTTGCGTTCGCCACCTGGAAACAGGCTGG
AAGCACTGTACGGTGACAGAGAGGGGCAGCACAGCATCCGAATCAATGGTCAATTCCGGATCTGTTTCATTTGGGGGCCT
AACGGTCCGCAAAACGTTGAAATTGTGGATTACCACTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT217240 WP_004026357.1 NZ_CP083752:31924-32367 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT217240 NZ_CP114115:1487713-1488018 [Pseudomonas sp. GXZC]
ATGTTTAAAAACGGCATGCGCCCCGTACACCCTGGAGAGATTCTCAAGGAAGAATACTTGGAGCCGTTGGGCCTTACGGC
TGCAGCCCTCGCGCGAGCGCTGCATGTCTCCGCGCCGACGGTGAATGACATTGTGCTGATGCGCCGTAGCATCAGTGCTG
ATGTGGCGCTTCGCTTGGCCGCTGCGTTGGAAACATCCGCGCAGTTTTGGCTTAACCTGCAGACCGCTTATGATTTGCGT
ACGGTGGAAATTGCCAAGGGCGCTCAGATATCCAAGGAAGTGGAGAAAGTCGCGCATTGCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9