Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5158046..5158671 Replicon chromosome
Accession NZ_CP083751
Organism Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LBE00_RS24835 Protein ID WP_002882817.1
Coordinates 5158046..5158429 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LBE00_RS24840 Protein ID WP_004150355.1
Coordinates 5158429..5158671 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBE00_RS24820 (LBE00_24855) 5155412..5156314 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LBE00_RS24825 (LBE00_24860) 5156311..5156946 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LBE00_RS24830 (LBE00_24865) 5156943..5157872 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LBE00_RS24835 (LBE00_24870) 5158046..5158429 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LBE00_RS24840 (LBE00_24875) 5158429..5158671 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LBE00_RS24845 (LBE00_24880) 5158876..5159793 + 918 WP_012737090.1 alpha/beta hydrolase -
LBE00_RS24850 (LBE00_24885) 5159807..5160748 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LBE00_RS24855 (LBE00_24890) 5160793..5161230 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LBE00_RS24860 (LBE00_24895) 5161227..5162087 - 861 WP_004146232.1 virulence factor BrkB family protein -
LBE00_RS24865 (LBE00_24900) 5162081..5162680 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T217239 WP_002882817.1 NZ_CP083751:c5158429-5158046 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217239 NZ_CP114115:c789599-789291 [Pseudomonas sp. GXZC]
ATGATTCGATTCAAGAGATCCGACGAGTTTCAAGAGTGGCTTGATTCGTCACGCAGCAGGCCAGCCCGCGGTCGTGTGTT
GACAAGATTGGATAACGCAACGAGGGGTAACTTCGGAGACTGTGCACCCGTAGGAAATGGCGTATCAGAAATGCGCATCC
ACTATGGCCCTGGATACCGCGTTTACTTCACACGCGTTGACGAGGTGGTTTACCTGTTGTTGATAGGTGGCGATAAATCC
ACGCAAAAACGCGACATCCAGCGCGCTAAAGAAATAGCCGATGAATTTGGAATAAGGAGTGACTCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217239 WP_004150355.1 NZ_CP083751:c5158671-5158429 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217239 NZ_CP114115:c789294-789007 [Pseudomonas sp. GXZC]
ATGACTCAATTTAAAGACTTCGACATAGCTGAGCACCTCAAGACTCCCGAGGACATGGCCGAGTACCTTGAAGCTTCTTT
CGAAGAGGACTCCGGCGACGGCTTGCTGATTCGCTCAGCCCTGAACAACATCGCCCGCGCCCAGGGCATGACCCAAATCG
CCCGCGACGCCGGCCTCGGTCGCGAAAGCCTATACAAAGCCCTCTCCGCCACTGGCAATCCCGAATTCGCGACTATCATG
AAAGTCATGAAAGCGTTGGGCCTGAAGCTTCACGCCACCGCTATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References