Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4575118..4575928 Replicon chromosome
Accession NZ_CP083751
Organism Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LBE00_RS22105 Protein ID WP_004178461.1
Coordinates 4575118..4575651 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LBE00_RS22110 Protein ID WP_002887278.1
Coordinates 4575662..4575928 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBE00_RS22100 (LBE00_22130) 4573949..4575070 + 1122 WP_064153030.1 cupin domain-containing protein -
LBE00_RS22105 (LBE00_22135) 4575118..4575651 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LBE00_RS22110 (LBE00_22140) 4575662..4575928 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LBE00_RS22115 (LBE00_22145) 4576031..4577464 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
LBE00_RS22120 (LBE00_22150) 4577454..4578137 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
LBE00_RS22125 (LBE00_22155) 4578310..4579695 + 1386 WP_064153031.1 efflux transporter outer membrane subunit -
LBE00_RS22130 (LBE00_22160) 4579713..4580057 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T217236 WP_004178461.1 NZ_CP083751:c4575651-4575118 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T217236 NZ_CP114066:c1378081-1377335 [Bacillus halotolerans]
ATGCTTTTATTCTTTCAAATCATGGTCTGGTGCACTATGGCCGCTTTAGGCTTATACGTATACGCCACATGGCGGTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGAAAAACATGGTATTTGCTGTTTGTTTTGGGCGCCATGATATACTGGA
CATATGATCCCTCTTCCCTGTTCACAAACTGGGAACGGTATGTCATTGTCGCTGTCAGCTTTGCTTTAATTGACGCTTTT
ATCTTTTTGAGCGCTTATGTGAAAAAACTGGCCGGCAGTGAACTGGAAACAGACACAAGAGAAATTCTCGAAGAAAACAA
CGAAATGCTCCACATGTATCTCGATCGGCTGAAAACATACCAATATCTATTGAAAAACGAACCGATTCATGTTTATTATG
GAAGTATAGAAGCATATGCTGAAGGAATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAATCTGACGGCCAGCCTT
TGTTACTATTCGACACAGTCTGATAAAGACCGGTTAACCGAGCACATGGAGGATCCCGCTGACGTGCAAACGAGGCTGGA
TCGAAAGGATGTCTATTACGACCAGTATGGAAAAGTAGTGCTCATTCCCTTTACAATCGAGACACAGAGCTATGTCATCA
AACTGACGTCTGACAGCATTGTAACGGAATTTGATTATTTGCTATTCACATCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT217236 WP_002887278.1 NZ_CP083751:c4575928-4575662 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT217236 NZ_CP114066:c1377335-1377165 [Bacillus halotolerans]
ATGGAAAACGCGTTTCAAAACGGATGCCATCCGCGGGCAAAGAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGATGAGTATAAACGTA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References