Detailed information of TA system TA021723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-DinJ
• Location: 1054671..1055251
• Replicon: chromosome
• Accession: NC_008530
• Organism: Lactobacillus gasseri ATCC 33323

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A249NKC7
• Locus tag: LGAS_RS05150
• Protein ID: WP_003647258.1
• Coordinates: 1054946..1055251 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A249NIV0
• Locus tag: LGAS_RS05145
• Protein ID: WP_021314843.1
• Coordinates: 1054671..1054946 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGAS_RS05115	1050550..1051440	-	891	WP_003651609.1	ribose transporter RbsU	-
LGAS_RS05120	1051467..1051862	-	396	WP_003647263.1	D-ribose pyranase	-
LGAS_RS05125	1051971..1052420	-	450	WP_003647262.1	ASCH domain-containing protein	-
LGAS_RS05130	1052486..1053187	-	702	WP_011678901.1	nucleoside phosphorylase	-
LGAS_RS05135	1053314..1053802	-	489	WP_003647261.1	GNAT family N-acetyltransferase	-
LGAS_RS05140	1053805..1054338	-	534	WP_003647260.1	GNAT family N-acetyltransferase	-
LGAS_RS05145	1054671..1054946	+	276	WP_021314843.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LGAS_RS05150	1054946..1055251	+	306	WP_003647258.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LGAS_RS05155	1055414..1056229	-	816	WP_011678902.1	Fic family protein	-
LGAS_RS05160	1056374..1057162	-	789	WP_003647255.1	tyrosine-protein phosphatase	-
LGAS_RS05165	1057231..1057560	-	330	WP_003647254.1	hypothetical protein	-
LGAS_RS05170	1057535..1058254	-	720	WP_003647253.1	hypothetical protein	-
LGAS_RS05175	1058261..1058620	-	360	WP_003647252.1	hypothetical protein	-
LGAS_RS05180	1058613..1058915	-	303	WP_003647251.1	hypothetical protein	-
LGAS_RS05185	1058927..1059736	-	810	WP_003647250.1	DUF2785 domain-containing protein	-
LGAS_RS09475	1059739..1060186	-	448	Protein_1022	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-97)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-88)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11985.87 Da
• Isoelectric Point: 10.2029

>T21723 WP_003647258.1 NC_008530:1054946-1055251 [Lactobacillus gasseri ATCC 33323 = JCM 1131]
MAQINYTPQFKRDYKKLKRKHYDLNKLKNVIQLLIDEKFKILVDKYDDHQLKGSLRSLRALHVEHNKAGNWILVYKIHSG
NLELLTIDLVSTGSHDHTYRT

Nucleotide

• Length: 306 bp

>T21723 NC_008530:1054946-1055251 [Lactobacillus gasseri ATCC 33323 = JCM 1131]
ATGGCTCAAATTAACTACACTCCTCAATTTAAGCGAGATTATAAGAAGCTTAAAAGAAAACATTATGATTTGAATAAATT
AAAAAATGTCATTCAATTACTTATTGACGAAAAATTTAAAATTCTTGTTGATAAATATGATGACCATCAACTAAAAGGTT
CTTTAAGGTCACTACGTGCATTACATGTTGAGCATAATAAAGCTGGTAATTGGATTTTAGTTTATAAAATTCATAGCGGC
AATTTAGAACTGTTAACCATCGACTTGGTTTCTACTGGTTCCCACGATCATACTTATAGAACATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10461.79 Da
• Isoelectric Point: 4.8096

>AT21723 WP_021314843.1 NC_008530:1054671-1054946 [Lactobacillus gasseri ATCC 33323 = JCM 1131]
MTTTVSLRLPEDLKKQATDVFNEYGLDWSSAMRMILTQVVSENAIPVNLHRYSEISPSMKSNIDRSLQEYKAGNYKTTNS
VKELFEELDKD

Nucleotide

• Length: 276 bp

>AT21723 NC_008530:1054671-1054946 [Lactobacillus gasseri ATCC 33323 = JCM 1131]
ATGACAACTACCGTAAGTTTAAGATTACCAGAAGATTTAAAGAAACAAGCAACAGATGTATTCAACGAATACGGTCTCGA
TTGGTCATCTGCAATGCGAATGATCTTAACTCAAGTCGTAAGTGAAAATGCAATTCCTGTTAATTTACATAGATATTCTG
AAATTTCTCCTTCGATGAAATCAAATATTGATAGGTCACTTCAAGAATACAAAGCAGGAAATTATAAAACCACAAACTCA
GTAAAAGAGCTATTTGAGGAGTTAGATAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A249NKC7

Antitoxin

• AlphaFold DB: A0A249NIV0