Detailed information of TA system TA217222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199575..200314
• Replicon: chromosome
• Accession: NZ_CP083751
• Organism: Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LBE00_RS00980
• Protein ID: WP_021312536.1
• Coordinates: 199829..200314 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LBE00_RS00975
• Protein ID: WP_003026799.1
• Coordinates: 199575..199841 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBE00_RS00960 (LBE00_00960)	195078..197147	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
LBE00_RS00965 (LBE00_00965)	197444..198813	+	1370	WP_087830479.1	IS3 family transposase	-
LBE00_RS00970 (LBE00_00970)	199014..199442	+	429	WP_004901287.1	GFA family protein	-
LBE00_RS00975 (LBE00_00975)	199575..199841	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LBE00_RS00980 (LBE00_00980)	199829..200314	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
LBE00_RS00985 (LBE00_00985)	200658..200810	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
LBE00_RS00990 (LBE00_00990)	201112..202731	+	1620	WP_032415799.1	ABC-F family ATP-binding cassette domain-containing protein	-
LBE00_RS00995 (LBE00_00995)	202830..203042	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
LBE00_RS01000 (LBE00_01000)	203295..203585	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
LBE00_RS01005 (LBE00_01005)	203831..205186	-	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	198181..198813	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T217222 WP_021312536.1 NZ_CP083751:199829-200314 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T217222 NZ_CP114053:c1925026-1924799 [Bifidobacterium longum subsp. infantis]
ATGAAGAAGCTGGATCGTAATGTTGCCAAGAGAATCATCGCAAAGCTTAGGGAAATTTCACAGTTGGAAGATCCGAGAAG
CACCGGCAAGGCTCTTGCCGGTAATCTGGCAGGGCTGTGGCGTTACCGTGTCGGGGACTATCGTATCGTGTGCGATATAG
AGGATGAGGTTCTGCTGATTCTTGTGATTGATGTCGCGCACAGGAGCAAGGTGTACAAGCGTTGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT217222 WP_003026799.1 NZ_CP083751:199575-199841 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT217222 NZ_CP114053:c1925288-1925052 [Bifidobacterium longum subsp. infantis]
ATGGCCACGGTTACTGCAGCATTAAGGATGCCTGTCGAATTGGCGGCTCGTTACGATTGTCTGGCGAAAGCGACTGGTCG
TACGAAGACGTTTTACATGAACGAAGCCCTTACCGAATCGATCGATAGGTTTGAGTATGAATACGGCATTATGAAAAAGG
TCGAGGATTGGCGTGCCGGCAGACTCGAAACCGTGACGCTCGACGAGCTGGAGGAAAGCCTTGGCTTGGAGGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66