Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2406907..2407549 Replicon chromosome
Accession NZ_CP083749
Organism Bacillus sp. CRB-7

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag K8M08_RS12260 Protein ID WP_000635963.1
Coordinates 2406907..2407257 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8M08_RS12265 Protein ID WP_000004570.1
Coordinates 2407262..2407549 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M08_RS12245 (K8M08_12245) 2403843..2404301 - 459 WP_000344257.1 SprT family protein -
K8M08_RS12250 (K8M08_12250) 2404497..2404613 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8M08_RS12255 (K8M08_12255) 2404671..2406839 - 2169 WP_000426231.1 Tex family protein -
K8M08_RS12260 (K8M08_12260) 2406907..2407257 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8M08_RS12265 (K8M08_12265) 2407262..2407549 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8M08_RS12270 (K8M08_12270) 2407857..2409026 - 1170 WP_000390607.1 alanine racemase -
K8M08_RS12275 (K8M08_12275) 2409145..2410095 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
K8M08_RS12280 (K8M08_12280) 2410252..2410611 - 360 WP_000635047.1 holo-ACP synthase -
K8M08_RS12285 (K8M08_12285) 2410704..2411276 + 573 WP_003266133.1 rhomboid family intramembrane serine protease -
K8M08_RS12290 (K8M08_12290) 2411269..2412231 - 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T217221 WP_000635963.1 NZ_CP083749:c2407257-2406907 [Bacillus sp. CRB-7]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T217221 NZ_CP114053:1525944-1526267 [Bifidobacterium longum subsp. infantis]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGCCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT217221 WP_000004570.1 NZ_CP083749:c2407549-2407262 [Bacillus sp. CRB-7]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT217221 NZ_CP114053:1525718-1525957 [Bifidobacterium longum subsp. infantis]
ATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCACCCCGGC
CGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAAATGACG
TGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References