Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2541009..2541660 Replicon chromosome
Accession NC_008526
Organism Lactobacillus casei ATCC 334 (Lactobacillus paracasei ATCC 334)

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LSEI_RS12270 Protein ID WP_003567661.1
Coordinates 2541009..2541392 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LSEI_RS12275 Protein ID WP_003567665.1
Coordinates 2541412..2541660 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSEI_RS12265 2540304..2540825 - 522 WP_003567657.1 QueT transporter family protein -
LSEI_RS12270 2541009..2541392 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LSEI_RS12275 2541412..2541660 - 249 WP_003567665.1 antitoxin Antitoxin
LSEI_RS12280 2541728..2542864 - 1137 WP_003596242.1 alanine racemase -
LSEI_RS12285 2542851..2543225 - 375 WP_003567669.1 holo-ACP synthase -
LSEI_RS12290 2543395..2544903 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
LSEI_RS12295 2545204..2546592 - 1389 WP_011674931.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T21722 WP_003567661.1 NC_008526:c2541392-2541009 [Lactobacillus paracasei ATCC 334]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T21722 NC_008526:c2541392-2541009 [Lactobacillus paracasei ATCC 334]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCAGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT21722 WP_003567665.1 NC_008526:c2541660-2541412 [Lactobacillus paracasei ATCC 334]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT21722 NC_008526:c2541660-2541412 [Lactobacillus paracasei ATCC 334]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References