Detailed information of TA system TA217218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4397562..4398208
• Replicon: chromosome
• Accession: NZ_CP083746
• Organism: Pseudomonas sp. p1(2021b)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K8374_RS20510
• Protein ID: WP_196143798.1
• Coordinates: 4398026..4398208 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K8374_RS20505
• Protein ID: WP_224456974.1
• Coordinates: 4397562..4397969 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8374_RS20475 (K8374_20475)	4392564..4394231	-	1668	WP_224456968.1	gamma-glutamyltransferase	-
K8374_RS20480 (K8374_20480)	4394350..4395246	-	897	WP_224456969.1	LysR family transcriptional regulator	-
K8374_RS20485 (K8374_20485)	4395450..4396040	-	591	WP_224456970.1	NAD(P)H-dependent oxidoreductase	-
K8374_RS20490 (K8374_20490)	4396206..4396520	+	315	WP_224456971.1	putative quinol monooxygenase	-
K8374_RS20495 (K8374_20495)	4396689..4396958	-	270	WP_224456972.1	DUF3077 domain-containing protein	-
K8374_RS20500 (K8374_20500)	4396966..4397184	-	219	WP_224456973.1	hypothetical protein	-
K8374_RS20505 (K8374_20505)	4397562..4397969	-	408	WP_224456974.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K8374_RS20510 (K8374_20510)	4398026..4398208	-	183	WP_196143798.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8374_RS20515 (K8374_20515)	4398423..4399028	+	606	WP_224456975.1	CDP-alcohol phosphatidyltransferase family protein	-
K8374_RS20520 (K8374_20520)	4399059..4400816	+	1758	WP_224456976.1	bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase	-
K8374_RS20525 (K8374_20525)	4400806..4402245	+	1440	WP_224456977.1	phosphatase PAP2/dual specificity phosphatase family protein	-
K8374_RS20530 (K8374_20530)	4402242..4402670	+	429	WP_224456978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6891.97 Da
• Isoelectric Point: 10.8172

>T217218 WP_196143798.1 NZ_CP083746:c4398208-4398026 [Pseudomonas sp. p1(2021b)]
MRSRDVIQLIEADGWYEVEVKGSHHQFRHPIKRGRVTVPHPKSDLPKGTVHNILKQAGLK

Nucleotide

• Length: 183 bp

>T217218 NZ_CP114053:c970618-970316 [Bifidobacterium longum subsp. infantis]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14632.53 Da
• Isoelectric Point: 4.7169

>AT217218 WP_224456974.1 NZ_CP083746:c4397969-4397562 [Pseudomonas sp. p1(2021b)]
MKFPVVLHKDVDSDYGVIVPDVPGCFSAGASVAQALENVQEALTLHFEGLMADGETLPQAQEVDVHVANPDYAGGIWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELAAAH

Nucleotide

• Length: 408 bp

>AT217218 NZ_CP114053:c970319-970035 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAATACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATATGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAGATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAGACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA