Detailed information of TA system TA217216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2031815..2032436
• Replicon: chromosome
• Accession: NZ_CP083746
• Organism: Pseudomonas sp. p1(2021b)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2G8MXB6
• Locus tag: K8374_RS09535
• Protein ID: WP_016715573.1
• Coordinates: 2032254..2032436 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K8374_RS09530
• Protein ID: WP_224458809.1
• Coordinates: 2031815..2032216 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8374_RS09500 (K8374_09500)	2027011..2027409	+	399	WP_223625146.1	hypothetical protein	-
K8374_RS09505 (K8374_09505)	2027641..2028435	+	795	WP_224458805.1	DUF1376 domain-containing protein	-
K8374_RS09510 (K8374_09510)	2028432..2029205	+	774	WP_102082673.1	ATP-binding protein	-
K8374_RS09515 (K8374_09515)	2029202..2030614	+	1413	WP_224458806.1	replicative DNA helicase	-
K8374_RS09520 (K8374_09520)	2030601..2031101	+	501	WP_224458807.1	hypothetical protein	-
K8374_RS09525 (K8374_09525)	2031098..2031700	+	603	WP_224458808.1	hypothetical protein	-
K8374_RS09530 (K8374_09530)	2031815..2032216	-	402	WP_224458809.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K8374_RS09535 (K8374_09535)	2032254..2032436	-	183	WP_016715573.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8374_RS09540 (K8374_09540)	2032540..2032713	+	174	WP_224458810.1	hypothetical protein	-
K8374_RS09545 (K8374_09545)	2032926..2034017	+	1092	WP_224458811.1	hypothetical protein	-
K8374_RS09550 (K8374_09550)	2034055..2034375	+	321	WP_224458812.1	phage holin family protein	-
K8374_RS09555 (K8374_09555)	2034377..2034580	+	204	WP_224458813.1	hypothetical protein	-
K8374_RS09560 (K8374_09560)	2034577..2034768	+	192	WP_224458814.1	hypothetical protein	-
K8374_RS09565 (K8374_09565)	2034771..2035109	+	339	WP_224458815.1	HNH endonuclease signature motif containing protein	-
K8374_RS09570 (K8374_09570)	2035268..2035768	+	501	WP_224458816.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2016801..2056414	39613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6696.86 Da
• Isoelectric Point: 11.2336

>T217216 WP_016715573.1 NZ_CP083746:c2032436-2032254 [Pseudomonas sp. p1(2021b)]
VQSRQLIKELEAAGWVLKRVTGSHHIFKHPNNPNSIPVPHPKKDLPIGTVKSIKERAGLK

Nucleotide

• Length: 183 bp

>T217216 NZ_CP114053:c15260-14970 [Bifidobacterium longum subsp. infantis]
ATGAGCGGCATCCAGTACACGGTCAAGACCACGTCCAGATTCAGGAAGGACTTCAAACTCGCCAGACGACGCGGACTGGA
CGCCGGCCTTTTCAAACAGGTTGTATCCATTCTGTCCGAGGGCGGCACACTGCCCGACCGATATCATGACCATGCGCTTA
CAGGCAATATGACCGGCTTCAGGGAGTGCCATATCACACCAGACCTGCTGTTGGTCTATCTCATAGAGAAGGACGTGCTG
GTGCTCACTCTGACCCGAACGGGCACCCATAGTGATCTTTTCGGAAAATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14393.31 Da
• Isoelectric Point: 4.2585

>AT217216 WP_224458809.1 NZ_CP083746:c2032216-2031815 [Pseudomonas sp. p1(2021b)]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDTFEEAYAAAVEVAHIMLEEIAGSGQAIPMPSSAAAHRGNPDFADMGWGM
LEIDITPYLGKTEKVNVTLPGFVIQQIDRYVRDHNVKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT217216 NZ_CP114053:c15535-15257 [Bifidobacterium longum subsp. infantis]
ATGAGCGGAACGACAACCACCGTTAACGTCCGAATGAACGCTGAGCTGAAAAAACAAGCCGAGGAACTGTTCGAGGAGCT
GGGCATGAACCTGACCACCGCAGTCAACATATTCATCCGCCAATCCCTGCGACAGGGCGGCATACCGTTCAACGTCTCGT
TGGATGAGCCGAACAGGGAGACCGTCTCCGCCATGTTGGAGGCGAAGCGAATCGCAAGGGACGGGAACGTGAAGGGCTAC
CGGGACCTGAACGCCTTGTTCAAGGACTTGGACGCATGA

Structures

Toxin

• AlphaFold DB: A0A2G8MXB6