Detailed information of TA system TA217212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 239518..240135
• Replicon: chromosome
• Accession: NZ_CP083746
• Organism: Pseudomonas sp. p1(2021b)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K8374_RS01045
• Protein ID: WP_224457624.1
• Coordinates: 239518..239709 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K8374_RS01050
• Protein ID: WP_224457625.1
• Coordinates: 239728..240135 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8374_RS01035 (K8374_01035)	235954..237261	+	1308	WP_224457622.1	OprD family porin	-
K8374_RS01040 (K8374_01040)	237582..239246	-	1665	WP_224457623.1	FMN-binding glutamate synthase family protein	-
K8374_RS01045 (K8374_01045)	239518..239709	+	192	WP_224457624.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8374_RS01050 (K8374_01050)	239728..240135	+	408	WP_224457625.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K8374_RS01055 (K8374_01055)	240175..241560	-	1386	WP_224457626.1	NAD(P)/FAD-dependent oxidoreductase	-
K8374_RS01060 (K8374_01060)	241654..241857	-	204	WP_143515193.1	hypothetical protein	-
K8374_RS01065 (K8374_01065)	241925..242146	-	222	WP_224457627.1	DUF3079 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7134.29 Da
• Isoelectric Point: 10.9938

>T217212 WP_224457624.1 NZ_CP083746:239518-239709 [Pseudomonas sp. p1(2021b)]
VNSRELISPIEADGWYLVRSRGSHRHFKHSCKKGLVTIPHPKKDLLPATIASVLRQAQIRYAS

Nucleotide

• Length: 192 bp

>T217212 NZ_CP114035:c4860360-4860073 [Pseudomonas putida]
ATGACCTATAGCCTCGACTTCGATGCCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACCGTGCGTCAGCAGTTCAA
GAAGAAACTGGCAGAGGTCCTGCTAAACCCTCGAATCGAGGCAAACCGCCTTCACTCGCTGCCGGATTGCTACAAGATAA
AGCTTCGCAGCAGCGGGTTTAGGCTGGTCTATCAGGTCATTGACCAAGAGGTCGTGGTTTTTGTCGTCGCTGTCGATCGC
CGTGAAAGAGACCAAGCTTACAAAAAGGCTGCTGAGCGACTTGAGTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14703.76 Da
• Isoelectric Point: 4.4915

>AT217212 WP_224457625.1 NZ_CP083746:239728-240135 [Pseudomonas sp. p1(2021b)]
MLFPIAIFPGDDSHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHLELLAQDEQEIPKASKVTVHAANPTFAGCTWAL
VDIDITRYLGKAEKLNITLPAYLLNRIDAYVKNHPEQKSRSGFLAEAALKVLQGQ

Nucleotide

• Length: 408 bp

>AT217212 NZ_CP114035:c4860589-4860350 [Pseudomonas putida]
ATGGCTTCCATCAACATCCGCGTCGACGATGATCTCAAAGCCCGCGCCTACAAAGAGCTCGAGCGCCTCGGTGTAACCCC
CTCCGAACTCATGCGACAGGCTCTGCAATATGTCGCTGAGCGGGGCAAGCTACCCTTCAGGCCAGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCTACCGTACAAGAGCGACTTGCTTCTGCGCAGCGCGTGAGGGTCCAGCTGGATGACCTATAG