Detailed information of TA system TA217211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5097609..5098248
• Replicon: chromosome
• Accession: NZ_CP083741
• Organism: Mycobacterium ulcerans strain BS123

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: L7VCR2
• Locus tag: LAZ99_RS22935
• Protein ID: WP_015357076.1
• Coordinates: 5097802..5098248 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: LAZ99_RS22930
• Protein ID: WP_240274553.1
• Coordinates: 5097609..5097797 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAZ99_RS22915	5092786..5094538	+	1753	Protein_4530	PPE domain-containing protein	-
LAZ99_RS22920	5094562..5095863	+	1302	WP_240274422.1	ISAs1 family transposase	-
LAZ99_RS22925	5095960..5097507	+	1548	WP_240274552.1	hypothetical protein	-
LAZ99_RS22930	5097609..5097797	+	189	WP_240274553.1	antitoxin	Antitoxin
LAZ99_RS22935	5097802..5098248	+	447	WP_015357076.1	SRPBCC family protein	Toxin
LAZ99_RS22940	5098360..5099358	-	999	WP_238559441.1	EspA/EspE family type VII secretion system effector	-
LAZ99_RS22945	5099779..5100552	+	774	WP_012396160.1	VOC family protein	-
LAZ99_RS22950	5100623..5101057	+	435	WP_012396159.1	hypothetical protein	-
LAZ99_RS22955	5101063..5101536	-	474	WP_012396158.1	VOC family protein	-
LAZ99_RS22960	5101553..5103073	-	1521	WP_240274554.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5094562..5095863	1301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15861.43 Da
• Isoelectric Point: 8.6574

>T217211 WP_015357076.1 NZ_CP083741:5097802-5098248 [Mycobacterium ulcerans]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLPETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSIVSFDVHLGGPALFGPIGMVVAAALRSDINASLRNFVTVFAPPAAG

Nucleotide

• Length: 447 bp

>T217211 NZ_CP114035:4686448-4686741 [Pseudomonas putida]
ATGACTGAAACCTTCTCTGAATTCGATCCTGCCGCGTACCTCACTACACCTGAAGCAATCGCGCAGTTCATGACCGATGC
ACTTGAAACCGGCGATGCCAGCTATGTCGCTAAAGCTGTCGGCGTGGTTGCACGTGCCAAGGGCATGAGCGAACTGGCGA
AAGAGACGGGGCTGTCCCGAGAGCAGCTTTACAGGTCATTCAGCGAGCATGGCAACCCCACGCTGAAGTCGTTTCTGACC
GTGATGAAGGCATTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6555.33 Da
• Isoelectric Point: 4.7205

>AT217211 WP_240274553.1 NZ_CP083741:5097609-5097797 [Mycobacterium ulcerans]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQPN

Nucleotide

• Length: 189 bp

>AT217211 NZ_CP114035:4686153-4686455 [Pseudomonas putida]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGACCGGACTACGGGACTTAAGCGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCGGGCGACGGATCTCCGGTAGGCCATGGCGTGAGTGAGCTCAAAATTCACT
ATGGTCCCGGCTATCGGGTGTACTTCCATCAGACGGGCAGCACCTTCGTCATTCTGCTCTGTGGCGGCGACAAAAGCAGT
CAAAAGCGAGACATCAAGGTTGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Structures

Toxin

• AlphaFold DB: A0A1B4XZB0