Detailed information of TA system TA217179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1004055..1004869
• Replicon: chromosome
• Accession: NZ_CP083731
• Organism: Salmonella sp. A29-2

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A3G3DUX8
• Locus tag: K8W53_RS04855
• Protein ID: WP_000971653.1
• Coordinates: 1004055..1004582 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: M7S6I2
• Locus tag: K8W53_RS04860
• Protein ID: WP_000855694.1
• Coordinates: 1004579..1004869 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8W53_RS04825 (1000429)	1000429..1000827	+	399	Protein_942	cytoplasmic protein	-
K8W53_RS04830 (1001038)	1001038..1001256	+	219	Protein_943	hypothetical protein	-
K8W53_RS04835 (1001413)	1001413..1002081	+	669	WP_000445914.1	hypothetical protein	-
K8W53_RS04840 (1002108)	1002108..1002602	+	495	WP_000424946.1	hypothetical protein	-
K8W53_RS04845 (1002774)	1002774..1003430	-	657	WP_023225036.1	protein-serine/threonine phosphatase	-
K8W53_RS04850 (1003780)	1003780..1003982	+	203	Protein_947	IS5/IS1182 family transposase	-
K8W53_RS04855 (1004055)	1004055..1004582	-	528	WP_000971653.1	GNAT family N-acetyltransferase	Toxin
K8W53_RS04860 (1004579)	1004579..1004869	-	291	WP_000855694.1	DUF1778 domain-containing protein	Antitoxin
K8W53_RS04865 (1005140)	1005140..1005340	-	201	Protein_950	transposase	-
K8W53_RS04870 (1005581)	1005581..1005907	+	327	WP_000393295.1	hypothetical protein	-
K8W53_RS04875 (1006180)	1006180..1006527	-	348	WP_001555786.1	DUF1493 family protein	-
K8W53_RS04880 (1006512)	1006512..1006961	-	450	WP_023225038.1	hypothetical protein	-
K8W53_RS04885 (1007393)	1007393..1007836	-	444	WP_023225039.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
K8W53_RS04890 (1008294)	1008294..1008944	+	651	WP_072203970.1	type III secretion system transcriptional activator InvF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	invH / invF / invG / invE / invA / invB	1002774..1014257	11483
	-	inside	IScluster/Tn	-	-	1003806..1005340	1534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19043.87 Da
• Isoelectric Point: 9.7032

>T217179 WP_000971653.1 NZ_CP083731:c1004582-1004055 [Salmonella sp. A29-2]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGRHDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T217179 NZ_CP113951:7701603-7701854 [Rhodococcus sp. JS3073]
ATGCGAACCATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGTCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTATCCGGGAT
CTGGCTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.50 Da
• Isoelectric Point: 6.3133

>AT217179 WP_000855694.1 NZ_CP083731:c1004869-1004579 [Salmonella sp. A29-2]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTQMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT217179 NZ_CP113951:7701851-7702111 [Rhodococcus sp. JS3073]
GTGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CAACACACTACTCGAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAGG
CCTACTGGTCCCGGCGTATCACCGACGAGCACCGGCTGGTTTATAAAATTGTCGATGATCAGATCCGCATTGCTGCCTGC
CGTTACCACTACGGACACTGA

Structures

Toxin

• AlphaFold DB: A0A3G3DUX8

Antitoxin

• AlphaFold DB: A0A3V8SJE7