Detailed information of TA system TA217175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2758304..2758833
• Replicon: chromosome
• Accession: NZ_CP083728
• Organism: Staphylococcus aureus strain MR254

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K9B11_RS13780
• Protein ID: WP_000621175.1
• Coordinates: 2758471..2758833 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: K9B11_RS13775
• Protein ID: WP_000948331.1
• Coordinates: 2758304..2758474 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9B11_RS13745 (2753340)	2753340..2753900	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
K9B11_RS13750 (2754109)	2754109..2754588	+	480	WP_001287088.1	hypothetical protein	-
K9B11_RS13755 (2754581)	2754581..2756164	+	1584	WP_001294626.1	PH domain-containing protein	-
K9B11_RS13760 (2756151)	2756151..2756642	+	492	WP_001205910.1	PH domain-containing protein	-
K9B11_RS13765 (2756646)	2756646..2757005	+	360	WP_000581200.1	holo-ACP synthase	-
K9B11_RS13770 (2757071)	2757071..2758219	+	1149	WP_001281145.1	alanine racemase	-
K9B11_RS13775 (2758304)	2758304..2758474	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
K9B11_RS13780 (2758471)	2758471..2758833	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9B11_RS13785 (2759183)	2759183..2760184	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
K9B11_RS13790 (2760303)	2760303..2760629	+	327	WP_001052491.1	anti-sigma factor antagonist	-
K9B11_RS13795 (2760631)	2760631..2761110	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
K9B11_RS13800 (2761085)	2761085..2761855	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T217175 WP_000621175.1 NZ_CP083728:2758471-2758833 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T217175 NZ_CP113946:1323791-1324063 [Lactobacillus sp. ESL0677]
ATGTTAAATATTAAGGTTACAGCTAAGTTTAGAAAAGACGTCAAGCGGTGTAATAAGCAAGGGCTACCAATGACTCAACT
TAAGTTAGTTATTCATGAACTTGAACAAAATCATGTCCTGCCCTCTAATTATAAAAATCATGCTTTAGATGGAACTTACT
CTAATTATCTTGAATGCCATATAAAGCCAGATTGGTTGTTAATATATCAAATTACTCAAACAACTTTGGCACTTATTAGA
ACAGGCAGTCATGCAGAATTATTTAAAAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT217175 WP_000948331.1 NZ_CP083728:2758304-2758474 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT217175 NZ_CP113946:1323530-1323787 [Lactobacillus sp. ESL0677]
ATGAATAAAACTTTACAGGCCCGAATGGATGGTAAACTTATGGATGAAGCAGAAGGTATCTTAAAAGATTTAGGACTTAA
TCGTTCAACTGCCTTGAAAATTTTTTATCAACAAATTGTATGGAATAATGGTTTACCTTTTGCAGTTAAAAAGCCTGAAA
TACCAAATGAAGAAACGCTGGATGCGATGCATGAAGATTTGTCTAAGACCAAGACTTACGATAATTCGGATGAATTATGG
CATGACTTGGGTATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1