Detailed information of TA system TA217162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 121552..122328
• Replicon: chromosome
• Accession: NZ_CP083728
• Organism: Staphylococcus aureus strain MR254

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: K9B11_RS00780
• Protein ID: WP_000031108.1
• Coordinates: 122176..122328 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: K9B11_RS00775
• Protein ID: WP_001251224.1
• Coordinates: 121552..122151 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9B11_RS00755 (117468)	117468..118925	+	1458	WP_000649907.1	ABC transporter permease subunit	-
K9B11_RS00760 (118918)	118918..119640	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
K9B11_RS00765 (119791)	119791..120918	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
K9B11_RS00770 (120923)	120923..121393	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
K9B11_RS00775 (121552)	121552..122151	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
K9B11_RS00780 (122176)	122176..122328	+	153	WP_000031108.1	SAS053 family protein	Toxin
K9B11_RS00785 (123105)	123105..123500	+	396	WP_000901021.1	hypothetical protein	-
K9B11_RS00790 (123696)	123696..125081	+	1386	WP_000116224.1	class II fumarate hydratase	-
K9B11_RS00795 (125544)	125544..126365	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T217162 WP_000031108.1 NZ_CP083728:122176-122328 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T217162 NZ_CP113936:c21627-21337 [Acinetobacter sp. ESL0695]
ATGGTTAAAGTTGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGATATTGTGATTTATCTCAA
TATGGTTCTTGAAGACAATGATCCTGCTGAATTAGCTCATGCTCTTGGTGTCATTGCCAAAGCTAGAGGTATGACACAGA
TTGCCAAAGAAGCAGGAATTGGTCGTGAAGCGCTGTATAAAGCTCTTCGCCATGATTCTGCACCTCGTTTCGATACGATC
AATCGTGTAGTGAATGCGCTTGGCTTAAAAATTACGGTACAACATGTATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT217162 WP_001251224.1 NZ_CP083728:121552-122151 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT217162 NZ_CP113936:c21925-21629 [Acinetobacter sp. ESL0695]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACCCGAATTCGTTTGAA
TCGCCGATTAGATAAGGTTCAACGTGGCAATTGGGGTGACATTAAGCCACTTCAAGAAGGTGTATGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATTGTCATGCTTGGTGGTGGTGAAAAATCT
ACTCAGAGTAAAGACATTGATCGAGCAGTCAAACTGTCGAAAACATTGGAGAATTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4