Detailed information of TA system TA021716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 141481..141986
• Replicon: chromosome
• Accession: NC_008463
• Organism: Pseudomonas aeruginosa UCBPP-PA14

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: PA14_RS00630
• Protein ID: WP_003083773.1
• Coordinates: 141481..141762 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0H2ZJU8
• Locus tag: PA14_RS00635
• Protein ID: WP_003137009.1
• Coordinates: 141759..141986 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA14_RS00605 (PA14_01460)	136732..138081	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
PA14_RS00610 (PA14_01470)	138130..138816	+	687	WP_003083762.1	FadR family transcriptional regulator	-
PA14_RS00615 (PA14_01480)	138917..139651	+	735	WP_003110658.1	GntR family transcriptional regulator	-
PA14_RS00620 (PA14_01490)	139855..140241	+	387	WP_003083767.1	aegerolysin family protein	-
PA14_RS00625 (PA14_01500)	140273..141181	-	909	WP_003083769.1	LysR family transcriptional regulator	-
PA14_RS00630 (PA14_01510)	141481..141762	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
PA14_RS00635 (PA14_01520)	141759..141986	-	228	WP_003137009.1	CopG family ribbon-helix-helix protein	Antitoxin
PA14_RS00640 (PA14_01540)	142162..142785	-	624	WP_003137011.1	hypothetical protein	-
PA14_RS00645 (PA14_01550)	142886..143386	+	501	WP_003137013.1	LEA type 2 family protein	-
PA14_RS00650 (PA14_01560)	143458..143799	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
PA14_RS00655 (PA14_01580)	143881..145308	-	1428	WP_003137015.1	GABA permease	-
PA14_RS00660 (PA14_01600)	145477..146970	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T21716 WP_003083773.1 NC_008463:c141762-141481 [Pseudomonas aeruginosa UCBPP-PA14]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T21716 NC_008463:c141762-141481 [Pseudomonas aeruginosa UCBPP-PA14]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.56 Da
• Isoelectric Point: 5.5740

>AT21716 WP_003137009.1 NC_008463:c141986-141759 [Pseudomonas aeruginosa UCBPP-PA14]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHAEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT21716 NC_008463:c141986-141759 [Pseudomonas aeruginosa UCBPP-PA14]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A0H2ZJU8