Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 121862..122779 | Replicon | plasmid pDMB05-1 |
| Accession | NZ_CP083716 | ||
| Organism | Bacillus velezensis strain DMB05 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | I2HQ15 |
| Locus tag | LAZ96_RS17775 | Protein ID | WP_007407256.1 |
| Coordinates | 122033..122779 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | LAZ96_RS17770 | Protein ID | WP_003154807.1 |
| Coordinates | 121862..122032 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LAZ96_RS17720 (LAZ96_17720) | 117069..117581 | + | 513 | WP_017417605.1 | sigma-70 family RNA polymerase sigma factor | - |
| LAZ96_RS17725 (LAZ96_17725) | 117694..118245 | + | 552 | Protein_132 | terminase | - |
| LAZ96_RS17730 (LAZ96_17730) | 118356..118718 | + | 363 | WP_014721043.1 | hypothetical protein | - |
| LAZ96_RS17735 (LAZ96_17735) | 118731..119102 | + | 372 | WP_017417608.1 | XkdW family protein | - |
| LAZ96_RS17740 (LAZ96_17740) | 119107..119304 | + | 198 | WP_007610833.1 | XkdX family protein | - |
| LAZ96_RS17745 (LAZ96_17745) | 119361..120122 | + | 762 | WP_025649639.1 | hypothetical protein | - |
| LAZ96_RS17750 (LAZ96_17750) | 120173..120436 | + | 264 | WP_003154815.1 | hemolysin XhlA family protein | - |
| LAZ96_RS17755 (LAZ96_17755) | 120450..120713 | + | 264 | WP_003154813.1 | phage holin | - |
| LAZ96_RS17760 (LAZ96_17760) | 120727..121605 | + | 879 | WP_007407257.1 | N-acetylmuramoyl-L-alanine amidase | - |
| LAZ96_RS17765 (LAZ96_17765) | 121640..121765 | - | 126 | WP_003154809.1 | hypothetical protein | - |
| LAZ96_RS17770 (LAZ96_17770) | 121862..122032 | - | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| LAZ96_RS17775 (LAZ96_17775) | 122033..122779 | - | 747 | WP_007407256.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| LAZ96_RS17780 (LAZ96_17780) | 122883..123881 | - | 999 | WP_017417610.1 | inorganic phosphate transporter | - |
| LAZ96_RS17785 (LAZ96_17785) | 123894..124511 | - | 618 | WP_003154804.1 | DUF47 domain-containing protein | - |
| LAZ96_RS17790 (LAZ96_17790) | 124798..126114 | - | 1317 | WP_007610842.1 | amino acid permease | - |
| LAZ96_RS17795 (LAZ96_17795) | 126436..127386 | + | 951 | WP_007610844.1 | ring-cleaving dioxygenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..806695 | 806695 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29077.57 Da Isoelectric Point: 4.7755
>T217155 WP_007407256.1 NZ_CP083716:c122779-122033 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
Download Length: 747 bp
>T217155 NZ_CP113921:29102-29368 [Lactobacillus sp. ESL0731]
ATGATATTAAGCTGGACTGACGAATCATGGGATGACTATGTTTATTGGCAGAAGCAGGGAGATAAGAAAAAAATCCGGCG
GATTAATAAGTTAATCAAAGATATTGAACGGCATCCTTTTGAAGGACTTGGGAAGCCAGAGGGATTAAAGCATGATCTGT
CTGGGTACTGGTCGCGAAAGATAGATGCAAAAGATCGAATTATCTATAAGTGTTCGGTGAATGAGCTAGTTATCTATTCT
TGTAAAGACCATTATGATGATCATTAA
ATGATATTAAGCTGGACTGACGAATCATGGGATGACTATGTTTATTGGCAGAAGCAGGGAGATAAGAAAAAAATCCGGCG
GATTAATAAGTTAATCAAAGATATTGAACGGCATCCTTTTGAAGGACTTGGGAAGCCAGAGGGATTAAAGCATGATCTGT
CTGGGTACTGGTCGCGAAAGATAGATGCAAAAGATCGAATTATCTATAAGTGTTCGGTGAATGAGCTAGTTATCTATTCT
TGTAAAGACCATTATGATGATCATTAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT217155 WP_003154807.1 NZ_CP083716:c122032-121862 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT217155 NZ_CP113921:28828-29121 [Lactobacillus sp. ESL0731]
ATGGATGCGGTAAACTATAGTAATTTTAGAAAGCATTTGAAAGATTATTTTAAGCAAGTTAACGATCATAGTGAGCCATT
AATTGTGACTAATAAAGAGCCCGAAGATAATGTTGTGGTGATGAGTAAGCAAGAATATGATTCTTTAGTTGAAACTCTAG
ATATTGAGTCGAATAAATATTTAATGGCTAAAATTCGCAATGGCCAAAAACAGGTTGATGCAAATAGAGCTGTTCATCAT
GAATTAATTGATCCAGATTATGAGGTAGATACTGATGATATTAAGCTGGACTGA
ATGGATGCGGTAAACTATAGTAATTTTAGAAAGCATTTGAAAGATTATTTTAAGCAAGTTAACGATCATAGTGAGCCATT
AATTGTGACTAATAAAGAGCCCGAAGATAATGTTGTGGTGATGAGTAAGCAAGAATATGATTCTTTAGTTGAAACTCTAG
ATATTGAGTCGAATAAATATTTAATGGCTAAAATTCGCAATGGCCAAAAACAGGTTGATGCAAATAGAGCTGTTCATCAT
GAATTAATTGATCCAGATTATGAGGTAGATACTGATGATATTAAGCTGGACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|