Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 2473050..2473687 | Replicon | chromosome |
| Accession | NZ_CP083715 | ||
| Organism | Bacillus velezensis strain DMB05 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | LAZ96_RS12720 | Protein ID | WP_003156187.1 |
| Coordinates | 2473337..2473687 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | LAZ96_RS12715 | Protein ID | WP_003156188.1 |
| Coordinates | 2473050..2473331 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LAZ96_RS12695 (LAZ96_12695) | 2469415..2470014 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| LAZ96_RS12700 (LAZ96_12700) | 2470107..2470472 | + | 366 | WP_014304402.1 | holo-ACP synthase | - |
| LAZ96_RS12705 (LAZ96_12705) | 2470637..2471644 | + | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| LAZ96_RS12710 (LAZ96_12710) | 2471761..2472930 | + | 1170 | WP_017419224.1 | alanine racemase | - |
| LAZ96_RS12715 (LAZ96_12715) | 2473050..2473331 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| LAZ96_RS12720 (LAZ96_12720) | 2473337..2473687 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| LAZ96_RS12725 (LAZ96_12725) | 2473805..2474626 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| LAZ96_RS12730 (LAZ96_12730) | 2474631..2474996 | + | 366 | WP_007609584.1 | RsbT antagonist protein RsbS | - |
| LAZ96_RS12735 (LAZ96_12735) | 2474999..2475400 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| LAZ96_RS12740 (LAZ96_12740) | 2475412..2476419 | + | 1008 | WP_007410233.1 | PP2C family protein-serine/threonine phosphatase | - |
| LAZ96_RS12745 (LAZ96_12745) | 2476483..2476812 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| LAZ96_RS12750 (LAZ96_12750) | 2476809..2477291 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| LAZ96_RS12755 (LAZ96_12755) | 2477257..2478045 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| LAZ96_RS12760 (LAZ96_12760) | 2478045..2478647 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T217154 WP_003156187.1 NZ_CP083715:2473337-2473687 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T217154 NZ_CP113920:637333-637656 [Bifidobacterium sp. ESL0732]
GTGAGACGCGGTGAGATCTGGACGGTGCTCACCGACGGCTACGCGAGCAAACCGAGGCCGGTCATTATCATCCAGAACGA
CGAGGTTCCGGATTTTCAATCGGTTGTGATGTGTCTGCTGACTTCCTATGATGCCGGCGATATCGCGACACGGGTGCGCA
TCGAACCTAGCTCTGGCAATGGGCTCAACAAAGTGAGCTTTGCGATGACCGATAAGATCTTGTCAGTGGAGCGCAAGGTT
TTGGGGCGCAAAGTCGGCGTTCTCGAGGGCAAGACGATGCGCGACATCGATAAGAAACTGATGGTGGTGCTCGGACTGGT
CTGA
GTGAGACGCGGTGAGATCTGGACGGTGCTCACCGACGGCTACGCGAGCAAACCGAGGCCGGTCATTATCATCCAGAACGA
CGAGGTTCCGGATTTTCAATCGGTTGTGATGTGTCTGCTGACTTCCTATGATGCCGGCGATATCGCGACACGGGTGCGCA
TCGAACCTAGCTCTGGCAATGGGCTCAACAAAGTGAGCTTTGCGATGACCGATAAGATCTTGTCAGTGGAGCGCAAGGTT
TTGGGGCGCAAAGTCGGCGTTCTCGAGGGCAAGACGATGCGCGACATCGATAAGAAACTGATGGTGGTGCTCGGACTGGT
CTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT217154 WP_003156188.1 NZ_CP083715:2473050-2473331 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT217154 NZ_CP113920:637101-637346 [Bifidobacterium sp. ESL0732]
ATGACCACTCAACTTGCAACAAGGGTGGACGACCAGCAGGCCGCTTTGTTCAAGCGCACTGCTCAAAAGCTCGGTACCAC
GGCTGCCGACGCCCTGCGTATGTTCGTCGCCGCGTTCAACGACAACCAAGGCTTCCCCTACGATGTTCGGCTCAAGAGCC
AAGCGCCCGAGCCCTTCGCCACCGAGCAGGAAGCCACGGACTTCGCTTCCAAGATGGCGGGGAGGTTGCTCAGTGAGACG
CGGTGA
ATGACCACTCAACTTGCAACAAGGGTGGACGACCAGCAGGCCGCTTTGTTCAAGCGCACTGCTCAAAAGCTCGGTACCAC
GGCTGCCGACGCCCTGCGTATGTTCGTCGCCGCGTTCAACGACAACCAAGGCTTCCCCTACGATGTTCGGCTCAAGAGCC
AAGCGCCCGAGCCCTTCGCCACCGAGCAGGAAGCCACGGACTTCGCTTCCAAGATGGCGGGGAGGTTGCTCAGTGAGACG
CGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|