Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 101205..101731 Replicon plasmid unnamed1
Accession NZ_CP083710
Organism Enterobacter cloacae complex sp. ECL352

Toxin (Protein)


Gene name relE Uniprot ID A0A7R6HXL1
Locus tag LBF07_RS23790 Protein ID WP_015572079.1
Coordinates 101444..101731 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LBF07_RS23785 Protein ID WP_000534858.1
Coordinates 101205..101444 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBF07_RS23760 (LBF07_23760) 97145..98191 - 1047 WP_045325187.1 NAD(P)-dependent alcohol dehydrogenase -
LBF07_RS23765 (LBF07_23765) 98208..99425 - 1218 WP_072206003.1 MFS transporter -
LBF07_RS23770 (LBF07_23770) 99553..100038 - 486 WP_045325184.1 cyclophilin-like fold protein -
LBF07_RS23775 (LBF07_23775) 100131..101030 + 900 WP_045325182.1 LysR family transcriptional regulator -
LBF07_RS23780 (LBF07_23780) 101081..101180 - 100 Protein_104 hypothetical protein -
LBF07_RS23785 (LBF07_23785) 101205..101444 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LBF07_RS23790 (LBF07_23790) 101444..101731 + 288 WP_015572079.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LBF07_RS23800 (LBF07_23800) 101803..101961 + 159 WP_013087178.1 type I toxin-antitoxin system Hok family toxin -
LBF07_RS23805 (LBF07_23805) 102685..103293 + 609 WP_094935623.1 hypothetical protein -
LBF07_RS23810 (LBF07_23810) 103322..104110 + 789 WP_015572118.1 DsbA family protein -
LBF07_RS23815 (LBF07_23815) 104691..105308 + 618 Protein_110 plasmid replication initiator RepA -
LBF07_RS23820 (LBF07_23820) 105588..106592 - 1005 WP_000427614.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..165418 165418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.09 Da        Isoelectric Point: 10.0714

>T217153 WP_015572079.1 NZ_CP083710:101444-101731 [Enterobacter cloacae complex sp. ECL352]
MAYFLDFDERALKEWRKLGSTVREQLKNKLAEVLESPRIDANKLRGMHDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T217153 NZ_CP113919:150550-150846 [Bifidobacterium sp. ESL0764]
ATGCTGAATGCCTATTACACCTCGGCCTTCTCGCGTGATGTCAAGAAGATGAAGCGCAAGCATGAGGATAAGCATATCGA
AGATTTGAAGACTGTGGTTCGTCTGGTCCTGGCTGATACCGCTGAAGCAAAAGCGGAATTGCGCAGACGCCACCGGGCTC
ATCGCTTGACCGGTCAGTGGTCGGGAGTGCTTGAATGCCATATCGGCAACAGCGGTGATCGTCTATTGATATGGCAAACG
GAAGACGGTTCGGCGATCTTCGTGCGTGTAGGCAGTCATGATGAGCTGTTTAGTTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT217153 WP_000534858.1 NZ_CP083710:101205-101444 [Enterobacter cloacae complex sp. ECL352]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT217153 NZ_CP113919:150266-150538 [Bifidobacterium sp. ESL0764]
ATCAGGATGGCGACGGTAACGGTACGGGTCGATGAACAGACCAAGAAGATGGCGTCGGCGCTTGCCGAGGAGTTCGGTTT
CGATCTTTCGAGCGTCACCAGGGCTTTCTATAAGCAGATGATTCGTGAGCAGTCCATTCCGCTGAACCTGACGCATACCG
CTGTCCCGAACGAAGCGACGAAGGCGGCGTTGCGTGAGGCCTCGCAGATACATAAGTCGCAGGATCCTGGATTCTCCAGC
GCCGACGAGATGTTCAGCTCACTCGGCATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References