Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/ElaA-DUF1778 |
| Location | 45089..45828 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP083710 | ||
| Organism | Enterobacter cloacae complex sp. ECL352 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A411GR51 |
| Locus tag | LBF07_RS23480 | Protein ID | WP_032664832.1 |
| Coordinates | 45089..45574 (-) | Length | 162 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | A0A411GR70 |
| Locus tag | LBF07_RS23485 | Protein ID | WP_032664831.1 |
| Coordinates | 45562..45828 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LBF07_RS23440 (LBF07_23440) | 40642..40854 | - | 213 | WP_000993245.1 | DUF3330 domain-containing protein | - |
| LBF07_RS23445 (LBF07_23445) | 40920..41156 | - | 237 | WP_001087807.1 | broad-spectrum mercury transporter MerE | - |
| LBF07_RS23450 (LBF07_23450) | 41153..41518 | - | 366 | WP_001277466.1 | mercury resistance co-regulator MerD | - |
| LBF07_RS23455 (LBF07_23455) | 41536..43221 | - | 1686 | WP_043907010.1 | mercury(II) reductase | - |
| LBF07_RS23460 (LBF07_23460) | 43260..43685 | - | 426 | WP_000522996.1 | organomercurial transporter MerC | - |
| LBF07_RS23465 (LBF07_23465) | 43713..43988 | - | 276 | WP_000732275.1 | mercury resistance system periplasmic binding protein MerP | - |
| LBF07_RS23470 (LBF07_23470) | 44004..44369 | - | 366 | WP_001294656.1 | mercuric ion transporter MerT | - |
| LBF07_RS23475 (LBF07_23475) | 44441..44896 | + | 456 | WP_001166628.1 | Hg(II)-responsive transcriptional regulator | - |
| LBF07_RS23480 (LBF07_23480) | 45089..45574 | - | 486 | WP_032664832.1 | GNAT family N-acetyltransferase | Toxin |
| LBF07_RS23485 (LBF07_23485) | 45562..45828 | - | 267 | WP_032664831.1 | DUF1778 domain-containing protein | Antitoxin |
| LBF07_RS24645 | 47365..48273 | + | 909 | Protein_48 | plasmid replication initiator RepA | - |
| LBF07_RS23505 (LBF07_23505) | 49166..49969 | + | 804 | WP_032664829.1 | DUF4225 domain-containing protein | - |
| LBF07_RS23510 (LBF07_23510) | 49947..50270 | - | 324 | WP_032664828.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..165418 | 165418 | |
| - | flank | IS/Tn | - | - | 46609..47037 | 428 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 162 a.a. Molecular weight: 17593.31 Da Isoelectric Point: 9.6084
>T217152 WP_032664832.1 NZ_CP083710:c45574-45089 [Enterobacter cloacae complex sp. ECL352]
VGRVTAPEPLSSCHQLAEFASGETVLDDWLKQRGLKNQALGAARTFVVCKANTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKSFYLHHGFKPSQTQERTLFLKLP
Q
VGRVTAPEPLSSCHQLAEFASGETVLDDWLKQRGLKNQALGAARTFVVCKANTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKSFYLHHGFKPSQTQERTLFLKLP
Q
Download Length: 486 bp
>T217152 NZ_CP113918:1078028-1078318 [Bifidobacterium sp. ESL0769]
GTGGCACTGCAACGAGTTGAGCGTACGTCGAAGTTTTTACGGCAGGCAAAGCAGCTTCAACGTAAGCATTATGATTTTGA
CGAATTGGAACATGTGATTCGTCTTTTGATGGAGCAGGACAAGGAAGTGCTGTACCGCCAATATCGTGACCATGCTTTGA
AAGGGAATCTACGGCGATTCCGCGAACTCCATATCGATGCTGATTGGTTGTTGATCTATGGAATCAAGCACGAAACCCTG
ACACTCATATTGGTGGAGACCGGTACTCATCGCCAGTTGTTGGGTAAATAA
GTGGCACTGCAACGAGTTGAGCGTACGTCGAAGTTTTTACGGCAGGCAAAGCAGCTTCAACGTAAGCATTATGATTTTGA
CGAATTGGAACATGTGATTCGTCTTTTGATGGAGCAGGACAAGGAAGTGCTGTACCGCCAATATCGTGACCATGCTTTGA
AAGGGAATCTACGGCGATTCCGCGAACTCCATATCGATGCTGATTGGTTGTTGATCTATGGAATCAAGCACGAAACCCTG
ACACTCATATTGGTGGAGACCGGTACTCATCGCCAGTTGTTGGGTAAATAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10077.50 Da Isoelectric Point: 4.4733
>AT217152 WP_032664831.1 NZ_CP083710:c45828-45562 [Enterobacter cloacae complex sp. ECL352]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADNVAIDKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADNVAIDKLL
ARKPQWDV
Download Length: 267 bp
>AT217152 NZ_CP113918:1077766-1078047 [Bifidobacterium sp. ESL0769]
ATGAGTTTCACTGCTAACGCGCCCAAGGCTACGCGAATTAATTTTCGTACCGATGAGGAGACAAAAGAGGAAGCAAATTC
AATTTTTGCCAAGCTTGGTTTAGATATGTCGACTGCGCTAAACATGTTTTTGCAACAGACTATCAACGATCAAGGGTTGC
CTTTCAAGCCGACGCTTTCCAAGTTCGAAAAAAGCGTACTGAAAGCAGCTGATGGTCCGACTCGTTCATTCGACAACGTT
GATGATTTGATGAAGGAGCTTCGTGGCACTGCAACGAGTTGA
ATGAGTTTCACTGCTAACGCGCCCAAGGCTACGCGAATTAATTTTCGTACCGATGAGGAGACAAAAGAGGAAGCAAATTC
AATTTTTGCCAAGCTTGGTTTAGATATGTCGACTGCGCTAAACATGTTTTTGCAACAGACTATCAACGATCAAGGGTTGC
CTTTCAAGCCGACGCTTTCCAAGTTCGAAAAAAGCGTACTGAAAGCAGCTGATGGTCCGACTCGTTCATTCGACAACGTT
GATGATTTGATGAAGGAGCTTCGTGGCACTGCAACGAGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A411GR51 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A411GR70 |