Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2894886..2895462 Replicon chromosome
Accession NZ_CP083709
Organism Enterobacter cloacae complex sp. ECL352

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag LBF07_RS14075 Protein ID WP_010427219.1
Coordinates 2894886..2895173 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag LBF07_RS14080 Protein ID WP_033144548.1
Coordinates 2895160..2895462 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBF07_RS14050 (LBF07_14050) 2889986..2890681 + 696 WP_094935517.1 winged helix-turn-helix domain-containing protein -
LBF07_RS14055 (LBF07_14055) 2890832..2891302 + 471 WP_094935516.1 MarR family transcriptional regulator -
LBF07_RS14060 (LBF07_14060) 2891299..2892366 + 1068 WP_094935515.1 HlyD family secretion protein -
LBF07_RS14065 (LBF07_14065) 2892371..2893447 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
LBF07_RS14070 (LBF07_14070) 2893444..2894715 - 1272 WP_094935514.1 DUF445 domain-containing protein -
LBF07_RS14075 (LBF07_14075) 2894886..2895173 + 288 WP_010427219.1 BrnT family toxin Toxin
LBF07_RS14080 (LBF07_14080) 2895160..2895462 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
LBF07_RS14085 (LBF07_14085) 2895493..2896131 - 639 WP_094935513.1 LysE family translocator -
LBF07_RS14090 (LBF07_14090) 2896179..2896922 - 744 WP_048976422.1 AraC family transcriptional regulator -
LBF07_RS14095 (LBF07_14095) 2897074..2898444 + 1371 WP_094935512.1 NAD-dependent succinate-semialdehyde dehydrogenase -
LBF07_RS14100 (LBF07_14100) 2898491..2898811 - 321 WP_039262264.1 hypothetical protein -
LBF07_RS14105 (LBF07_14105) 2898811..2899356 - 546 WP_023310269.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T217150 WP_010427219.1 NZ_CP083709:2894886-2895173 [Enterobacter cloacae complex sp. ECL352]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T217150 NZ_CP113918:171794-172057 [Bifidobacterium sp. ESL0769]
GTGACGTACCACGTTGTAATCAAGAACTCGGCCAAGTCCGATCTTAAAAAACTTAAGCGTTCACATCTTCAGCAATCATT
CATGCAGGTAGTCGCAGATCTGAAGTCTGATCCTTACCAGCCGACTCAATCATTCGAAAAGCTACAGCCCGTTAGCAGGA
GATTGTATTCCCGACGGATTAACAGCCAACATCGTGTTGTTTACGATATCGATGACGATACGCAGACAGTAACGATATAT
GCGGCTTGGAGTCATTACGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT217150 WP_033144548.1 NZ_CP083709:2895160-2895462 [Enterobacter cloacae complex sp. ECL352]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT217150 NZ_CP113918:171534-171797 [Bifidobacterium sp. ESL0769]
ATGGATACTTATACACCTACGGCGGCAAGGAAGAACCTGTACCAGATTCTCAAGGATGTCAATGTCCAGCGTAAACCAGT
AGTGATTTCTCCAGCAAGAGGCAACAAGGACGAGGAAGCAGTGGTGGTCAATCGTGCAGATTGGAACTCGATCATGGAAA
CCCTCTATCTGGAGAACACGGGCGCTCTTGCAACGGCTGAAAAACGTCGCGCTGATGACAGCGGAACCACGGATATCGAT
GATATCGATTGGGATACGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References