Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 2279321..2279937 Replicon chromosome
Accession NZ_CP083709
Organism Enterobacter cloacae complex sp. ECL352

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LBF07_RS11140 Protein ID WP_094935277.1
Coordinates 2279321..2279692 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag LBF07_RS11145 Protein ID WP_094935276.1
Coordinates 2279695..2279937 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBF07_RS11125 (LBF07_11125) 2276821..2277723 + 903 WP_010436855.1 formate dehydrogenase subunit beta -
LBF07_RS11130 (LBF07_11130) 2277720..2278355 + 636 WP_010436852.1 formate dehydrogenase cytochrome b556 subunit -
LBF07_RS11135 (LBF07_11135) 2278352..2279281 + 930 WP_023309751.1 formate dehydrogenase accessory protein FdhE -
LBF07_RS11140 (LBF07_11140) 2279321..2279692 - 372 WP_094935277.1 type II toxin-antitoxin system VapC family toxin Toxin
LBF07_RS11145 (LBF07_11145) 2279695..2279937 - 243 WP_094935276.1 CopG family transcriptional regulator Antitoxin
LBF07_RS11150 (LBF07_11150) 2280137..2281057 + 921 WP_094935275.1 alpha/beta hydrolase -
LBF07_RS11155 (LBF07_11155) 2281066..2282007 - 942 WP_039264191.1 fatty acid biosynthesis protein FabY -
LBF07_RS11160 (LBF07_11160) 2282052..2282489 - 438 WP_008501836.1 D-aminoacyl-tRNA deacylase -
LBF07_RS11165 (LBF07_11165) 2282486..2283367 - 882 WP_033146965.1 virulence factor BrkB family protein -
LBF07_RS11170 (LBF07_11170) 2283361..2283960 - 600 WP_094935274.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13680.87 Da        Isoelectric Point: 7.3604

>T217149 WP_094935277.1 NZ_CP083709:c2279692-2279321 [Enterobacter cloacae complex sp. ECL352]
MERMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFSGITGVVSPYQL

Download         Length: 372 bp

>T217149 NZ_CP113917:c2254085-2253951 [Bifidobacterium sp. ESL0775]
ATGCAGGTAGTGGCAGATCTGAAATCTGACCCGTATCAGCCGACCCAATCATTTGAAAAACTGCAGCCTGCTAGCCGGGG
ATTGTATTCCCGCCGGATCAATAGCCAGCATCGGGTGGTCTATGACCATCAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9455.65 Da        Isoelectric Point: 4.2803

>AT217149 WP_094935276.1 NZ_CP083709:c2279937-2279695 [Enterobacter cloacae complex sp. ECL352]
MILSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT217149 NZ_CP113917:c2254426-2254163 [Bifidobacterium sp. ESL0775]
ATGGATACTTATACTCCGACAGCAGCAAGGAAGAACCTGTACCAGATTCTCAAGGATGTCAACGTTCAGCGGAAACCCGT
AGTGATTTCTCCCGCAAGGGGCAACAAGGACGAGGAAGCCGTAGTCGTCAATCGTGCCGATTGGAATTCGATGATCGAAA
CCTTGTATCTGGAGAACACGGGCACACTCGCCACAGCTGAAAAACGCCGTGCTGACGATAGCGGAATCACGGATTTCGAT
GATATTGATTGGGATACGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References