Detailed information of TA system TA217125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 100636..101279
• Replicon: plasmid p1606A
• Accession: NZ_CP083702
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K9O95_RS25185
• Protein ID: WP_224451506.1
• Coordinates: 100863..101279 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: K9O95_RS25180
• Protein ID: WP_001261286.1
• Coordinates: 100636..100866 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O95_RS25160 (97267)	97267..98022	-	756	WP_000852146.1	replication initiation protein RepE	-
K9O95_RS25165 (98744)	98744..99550	-	807	WP_000016970.1	site-specific integrase	-
K9O95_RS25170 (99551)	99551..99856	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
K9O95_RS25175 (99858)	99858..100076	-	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	-
K9O95_RS25180 (100636)	100636..100866	+	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9O95_RS25185 (100863)	100863..101279	+	417	WP_224451506.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9O95_RS25190 (101354)	101354..102919	+	1566	WP_001128474.1	AAA family ATPase	-
K9O95_RS25195 (102904)	102904..103926	+	1023	WP_000361402.1	helicase UvrD	-
K9O95_RS25200 (104180)	104180..104866	-	687	Protein_123	IS1 family transposase	-
K9O95_RS25210 (105913)	105913..106026	+	114	Protein_125	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / tet(A)	-	1..120731	120731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15264.72 Da
• Isoelectric Point: 6.8536

>T217125 WP_224451506.1 NZ_CP083702:100863-101279 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHMQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T217125 NZ_CP113837:c4430685-4430422 [Kitasatospora sp. YST-16]
GTGAGTGAGTACCGAACCGTCTTCCACCCCGAGGCGCAGGCCGAACTTCGGAAGATCCCTCGCGACATGGCGCTGCGCAT
CCTGGCCAAGCTGGCCGAGTTGGAGACCGACCCTCTCGGCTTCAACACCACCGCACTCGTGTCCCAGCCCGAACGTCGCC
GCTTGCGCGTCGGCGACTACCGCGTCGTCTACACGATCGACAACGGGGAACTGGTGGTCTGGGTTGTTCACGTGGGGCAC
CGGTCCACGGTTTACGAGACCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT217125 WP_001261286.1 NZ_CP083702:100636-100866 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT217125 NZ_CP113837:c4430947-4430678 [Kitasatospora sp. YST-16]
ATGACGCAGCCTCTGCCCATAGAGTCCATTCGAGACGTGCGCGCACACCTCGCCGAGGTCGTGGAGCGCGCCGACCGCGA
CGACGTACCCACGGTGATCACGCGCCGCGGCAAGGAGGTCGCCGCCGTCGTCTCCATCGAGGTACTGCGCAAGTACCAGG
AGTGGGAAGAGCGCGAGATCAATCGCATCATCGACGAGCGCATGGCCAACCCGGCACCCGGAATCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCAGTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CKZ6